Chemerin Polyclonal antibody

Chemerin Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10216-1-AP

Synonyms

Chemerin, HP10433, RAR responsive protein TIG2, RARRES2, TIG2



Tested Applications

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman placenta tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

10216-1-AP targets Chemerin in WB, IHC,ELISA applications and shows reactivity with human, Mouse samples.

Tested Reactivity human, Mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Chemerin fusion protein Ag0261
Full Name retinoic acid receptor responder (tazarotene induced) 2
Calculated molecular weight 163 aa, 19 kDa
Observed molecular weight 14-19 kDa
GenBank accession numberBC000069
Gene symbol RARRES2
Gene ID (NCBI) 5919
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Chemerin, also named as TIG2 and RARRES2, is adipocyte-secreted protein (adipokine) that regulates adipogenesis, metabolism and inflammation through activation of the chemokine-like receptor 1 (CMKLR1). Its other ligands include G protein-coupled receptor 1 (GPR1) and chemokine receptor-like 2 (CCRL2). It positively regulates adipocyte differentiation, modulates the expression of adipocyte genes involved in lipid and glucose metabolism and might play a role in angiogenesis, a process essential for the expansion of white adipose tissue. RARRES2 have both pro- and anti-inflammatory properties depending on the modality of enzymatic cleavage by different classes of proteases. The MW of Chemerin is 14-19 kDa.

Protocols

Product Specific Protocols
WB protocol for Chemerin antibody 10216-1-APDownload protocol
IHC protocol for Chemerin antibody 10216-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Zhejiang Univ Sci B

Silencing of DsbA-L gene impairs the PPARγ agonist function of improving insulin resistance in a high-glucose cell model.

Authors - Xuan Zhou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Owen (Verified Customer) (10-07-2019)

The antibody manages to detect the correct band of the protein and is specific. Further optimization for IHC would be great.

  • Applications: Western Blot,Immunofluorescence,
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: 786-O cell