|Positive WB detected in||human plasma tissue|
|Positive IHC detected in||human placenta tissue, mouse liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10216-1-AP targets Chemerin in WB, IHC,ELISA applications and shows reactivity with human, Mouse samples.
|Tested Reactivity||human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Chemerin fusion protein Ag0261|
|Full Name||retinoic acid receptor responder (tazarotene induced) 2|
|Calculated molecular weight||163 aa, 19 kDa|
|Observed molecular weight||14-19 kDa|
|GenBank accession number||BC000069|
|Gene ID (NCBI)||5919|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Chemerin, also named as TIG2 and RARRES2, is adipocyte-secreted protein (adipokine) that regulates adipogenesis, metabolism and inflammation through activation of the chemokine-like receptor 1 (CMKLR1). Its other ligands include G protein-coupled receptor 1 (GPR1) and chemokine receptor-like 2 (CCRL2). It positively regulates adipocyte differentiation, modulates the expression of adipocyte genes involved in lipid and glucose metabolism and might play a role in angiogenesis, a process essential for the expansion of white adipose tissue. RARRES2 have both pro- and anti-inflammatory properties depending on the modality of enzymatic cleavage by different classes of proteases. The MW of Chemerin is 14-19 kDa.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Owen (Verified Customer) (10-07-2019)
The antibody manages to detect the correct band of the protein and is specific. Further optimization for IHC would be great.