Tested Applications
| Positive WB detected in | HL-60 cells, human placenta tissue, mouse placenta tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16079-1-AP targets RARS2 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9074 Product name: Recombinant human RARS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 230-578 aa of BC010420 Sequence: LELGDVQALSLWQKFRDLSIEEYIRVYKRLGVYFDEYSGESFYREKSQEVLKLLESKGLLLRTIKGTAVVDLSGNGDPSSICTVMRSDGTSLYATRDLAAAIDRMDKYNFDTMIYVTDKGQKKHFQQVFQMLKIMGYDWAERCQHVPFGVVQGMKTRRGDVTFLEDVLNEIQLRMLQNMASIKTTKELKNPQETAERVGLAALIIQDFKGLLLSDYKFSWDRVFQSRGDTGVFLQYTHARLHSLEETFGCGYLNDFNTACLQEPQSVSILQHLLRFDEVLYKSSQDFQPRHIVSYLLTLSHLAAVAHKTLQIKDSPPEVAGARLHLFKAVRSVLANGMKLLGITPVCRM Predict reactive species |
| Full Name | arginyl-tRNA synthetase 2, mitochondrial |
| Calculated Molecular Weight | 578 aa, 66 kDa |
| Observed Molecular Weight | 66-70 kDa |
| GenBank Accession Number | BC010420 |
| Gene Symbol | RARS2 |
| Gene ID (NCBI) | 57038 |
| RRID | AB_3669230 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5T160 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RARS2 encodes the mitochondrial arginyl transfer RNA (tRNA) synthetase, an enzyme which belongs to the group of mitochondrial aminoacyl tRNA synthetases. These nuclear encoded mitochondrial proteins play a key role in mitochondrial protein translation by catalyzing the attachment of amino acids to their cognate tRNA molecules . Variants in RARS2 gene are associated with pontocerebellar hypoplasia type 6 (PCH6; MIM 611523), a rare mitochondrial encephalopathy with phenotype characterized by early onset seizures, profound developmental delay, and progressive pontocerebellar atrophy.(PMID: 27769281, PMID: 33209735)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RARS2 antibody 16079-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for this product rates it 4 out of 5 stars. The user performed a Western Blot on fibroblasts at a 1:1000 dilution and reported that the antibody works well overall. However, they noted that the protein ladder was also strongly recognized by the antibody, suggesting some non-specific binding to ladder components.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Vera (Verified Customer) (07-14-2026) | The antibody works well; however, the protein ladder is also strongly recognized by the antibody.
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