Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human testis tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12935-1-AP targets RASGAP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3906 Product name: Recombinant human RASA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-330 aa of BC033015 Sequence: MMAAEAGSEEGGPVTAGAGGGGAAAGSSAYPAVCRVKIPAALPVAAAPYPGLVETGVAGTLGGGAALGSEFLGAGSVAGALGGAGLTGGGTAAGVAGAAAGVAGAAVAGPSGDMALTKLPTSLLAETLGPGGGFPPLPPPPYLPPLGAGLGTVDEGDSLDGPEYEEEEVAIPLTAPPTNQWYHGKLDRTIAEERLRQAGKSGSYLIRESDRRPGSFVLSFLSQMNVVNHFRIIAMCGDYYIGGRRFSSLSDLIGYYSHVSCLLKGEKLLYPVAPPEPVEDRRRVRAILPYTKVPDTDEISFLKGDMFIVHNELEDGWMWVTNLRTDEQGL Predict reactive species |
| Full Name | RAS p21 protein activator (GTPase activating protein) 1 |
| Calculated Molecular Weight | 1047 aa, 116 kDa |
| Observed Molecular Weight | 120 kDa, 95 kDa |
| GenBank Accession Number | BC033015 |
| Gene Symbol | RASA1 |
| Gene ID (NCBI) | 5921 |
| RRID | AB_2175906 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20936 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RASGAP antibody 12935-1-AP | Download protocol |
| IHC protocol for RASGAP antibody 12935-1-AP | Download protocol |
| IP protocol for RASGAP antibody 12935-1-AP | Download protocol |
| WB protocol for RASGAP antibody 12935-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RASA1 antibody (Cat# 12935-1-AP) has 10 total citations published in journals including Cancer Research, Aging, Cell Signaling, Functional & Integrative Genomics, BMC Genomics, PLoS ONE, Biochemical & Biophysical Research Communications, American Journal of Translational Research, International Journal of Clinical and Experimental Pathology, and Bio Protocols. Research fields span oncology (melanoma, breast cancer, gastric cancer, osteosarcoma), vascular biology, developmental biology, subretinal fibrosis, and ischemia-reperfusion injury.
| Species | Application | Title |
|---|---|---|
Cancer Res Blocking Nitrosylation Induces Immunogenic Cell Death by Sensitizing NRAS-Mutant Melanoma to MEK Inhibitors | ||
Aging (Albany NY) LncRNA-HAGLR motivates triple negative breast cancer progression by regulation of WNT2 via sponging miR-335-3p. | ||
Cell Signal siRASA1 in vascular endothelial cells alleviates subretinal fibrosis through inhibiting macrophage-myofibroblast transdifferentiation via SHH signaling pathway.
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Funct Integr Genomics Amlodipine inhibits Synaptotagmin-4's oncogenic activity on gastric cancer proliferation by targeting calcium signaling | ||
BMC Genomics RASA1 expression highlights Z/W dosage dynamics and gonadal development in chickens.
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PLoS One Daraxonrasib, a pan-RAS inhibitor, selectively inhibits osteosarcomas with activated KRAS by halting AKT signaling and matrix metalloprotease activity.
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