RASAL2 Polyclonal antibody

• Featured Product
• KD/KO Validated

RASAL2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 22140-1-AP

Synonyms

nGAP, RAS protein activator like 2, RASAL2

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Validation Data Gallery

• 

  WB analysis of HeLa using 22140-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22140-1-AP (RASAL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 22140-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22140-1-AP (RASAL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

The immunogen of 22140-1-AP is RASAL2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RASAL2 fusion protein Ag17555
• Full Name: RAS protein activator like 2
• Calculated Molecular Weight: 1262 aa, 142 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC110611
• Gene Symbol: RASAL2
• Gene ID (NCBI): 9462
• RRID: AB_2879005
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RASAL2 (Ras protein activator like 2), also known as nGAP, is a Ras GTPase-activating protein, containing a GRD domain and a coiled-coil structure at the C terminus. It is able to complement the defective RasGAP function in a yeast system. RASAL2 is highly homologous to Ras-specific GTPase activating proteins (RasGAPs). Two transcript variants exist for RASAL2 due to alternative splicing events.

Publications

Species	Application	Title
mouse	WB	Mol Metab; Rasal2 deficiency reduces adipogenesis and occurrence of obesity-related disorders.; Authors - Xiaoqiang Zhu ; KD Validated
human,mouse	WB,IHC,IF	J Clin Transl Hepatol; RASAL2 Deficiency Attenuates Hepatic Steatosis by Promoting Hepatic VLDL Secretion via the AKT/TET1/MTTP Axis; Authors - Hao Ding ; KD Validated
human	WB,IP,IHC,IF	Int J Biol Sci; RASAL2 mediated the enhancement of YAP1/TIAM1 signaling promotes malignant phenotypes of pancreatic ductal adenocarcinoma; Authors - Yangyang Yue ; KD Validated
human	IF	Transl Res; RAS protein activator-like 2 (RASAL2) initiates peritubular capillary rarefaction in hypoxic renal interstitial fibrosis; Authors - Yu Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

anwesh (Verified Customer) (11-14-2019)

Clean bands observed at the right size of ~140KD in both human and mouse cell lines

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: A549, MEF's cells

RASAL2 Antibody Western Blot, validation (1:1000 dilution) in A549, MEF's cells (Cat no:22140-1-AP)