RASGRP3 Polyclonal antibody

• Featured Product
• KD/KO Validated

RASGRP3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 13162-1-AP

Synonyms

GRP3, KIAA0846, Ras guanyl releasing protein 3, RAS-GRP, RASGRP3

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Validation Data Gallery

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  WB analysis using 13162-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13162-1-AP (RASGRP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 13162-1-AP

  WB result of RASGRP3 antibody (13162-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RASGRP3 transfected PC-3 cells.

• 

  WB analysis of PC-3 using 13162-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13162-1-AP (RASGRP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of RAW 264.7 using 13162-1-AP

  IP result of anti-RASGRP3 (IP:13162-1-AP, 4ug; Detection:13162-1-AP 1:500) with RAW 264.7 cells lysate 3200 ug.

• 

  IHC staining of human ovary tumor using 13162-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor slide using 13162-1-AP (RASGRP3 Antibody) at dilution of 1:50.

• 

  IHC staining of human ovary tumor using 13162-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor slide using 13162-1-AP (RASGRP3 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, PC-3 cells, Raji cells, Ramos cells
• Positive IP detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 5 publications below
• FC: See 1 publications below

Product Information

13162-1-AP targets RASGRP3 in WB, IP, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RASGRP3 fusion protein Ag3810
• Full Name: RAS guanyl releasing protein 3 (calcium and DAG-regulated)
• Calculated Molecular Weight: 690 aa, 78 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC027849
• Gene Symbol: RASGRP3
• Gene ID (NCBI): 25780
• RRID: AB_10640522
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AS guanyl releasing protein 3 (RasGRP3) is a member of the RasGRP family and is able to promote guanine nucleotide exchange of Ha-Ras, R-Ras and Rap1 (PMID: 9582122). RasGRP3 is involved in a diverse range of important biological processes. RasGRP3 has emerged as an important mediator of signaling downstream from receptor coupled phosphoinositide turnover in B and T cells. Overexpression of RasGRP3 is commonly associated with many malignancies, such as pre-Bcell leukemia, Burkitt's lymphoma and natural killer-like T-cell leukemia. Recently, it has been reported that upregulation of RASGRP3 expression in prostate cancer correlates with aggressive capabilities and predicts biochemical recurrence after radical prostatectomy (PMID: 24418912).

Publications

Species	Application	Title
human	IHC	Cancer Cell; RasGRP3 Mediates MAPK Pathway Activation in GNAQ Mutant Uveal Melanoma.; Authors - Xu Chen
human	WB,FC	BMC Med; Downregulation of AC092894.1 promotes oxaliplatin resistance in colorectal cancer via the USP3/AR/RASGRP3 axis; Authors - Zhijian Zheng ; KD Validated
human	WB,IHC	Am J Cancer Res; A somatic mutation of RasGRP3 decreases Na+/I- symporter expression in metastases of radioactive iodine-refractory thyroid cancer by stimulating the Akt signaling pathway.; Authors - Jianlu Song
human	WB,IHC	Gene; RasGRP3 controls cell proliferation and migration in papillary thyroid cancer by regulating the Akt-MDM2 pathway.; Authors - Wangwang Qiu ; KD Validated
human	WB, IHC	J Vet Sci; Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus.; Authors - Lishan Lv
human	WB,IHC	Prostate Cancer Prostatic Dis; Upregulation of RASGRP3 expression in prostate cancer correlates with aggressive capabilities and predicts biochemical recurrence after radical prostatectomy.; Authors - X Zeng