Tested Applications
| Positive WB detected in | RAW 264.7 cells, PC-3 cells, Raji cells, Ramos cells |
| Positive IP detected in | RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13162-1-AP targets RASGRP3 in WB, IHC, IP, IP-MS, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3810 Product name: Recombinant human RASGRP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 342-690 aa of BC027849 Sequence: SLQNASHHLEPNMDLINLLTLSLDLYHTEDDIYKLSLVLEPRNSKSQPTSPTTPNKPVVPLEWALGVMPKPDPTVINKHIRKLVESVFRNYDHDHDGYISQEDFESIAANFPFLDSFCVLDKDQDGLISKDEMMAYFLRAKSQLHCKMGPGFIHNFQEMTYLKPTFCEHCAGFLWGIIKQGYKCKDCGANCHKQCKDLLVLACRRFARAPSLSSGHGSLPGSPSLPPAQDEVFEFPGVTAGHRDLDSRAITLVTGSSRKISVRLQRATTSQATQTEPVWSEAGWGDSGSHTFPKMKSKFHDKAAKDKGFAKWENEKPRVHAGVDVVDRGTEFELDQDEGEETRQDGEDG Predict reactive species |
| Full Name | RAS guanyl releasing protein 3 (calcium and DAG-regulated) |
| Calculated Molecular Weight | 690 aa, 78 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC027849 |
| Gene Symbol | RASGRP3 |
| Gene ID (NCBI) | 25780 |
| RRID | AB_10640522 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IV61 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AS guanyl releasing protein 3 (RasGRP3) is a member of the RasGRP family and is able to promote guanine nucleotide exchange of Ha-Ras, R-Ras and Rap1 (PMID: 9582122). RasGRP3 is involved in a diverse range of important biological processes. RasGRP3 has emerged as an important mediator of signaling downstream from receptor coupled phosphoinositide turnover in B and T cells. Overexpression of RasGRP3 is commonly associated with many malignancies, such as pre-Bcell leukemia, Burkitt's lymphoma and natural killer-like T-cell leukemia. Recently, it has been reported that upregulation of RASGRP3 expression in prostate cancer correlates with aggressive capabilities and predicts biochemical recurrence after radical prostatectomy (PMID: 24418912).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RASGRP3 antibody 13162-1-AP | Download protocol |
| IP protocol for RASGRP3 antibody 13162-1-AP | Download protocol |
| WB protocol for RASGRP3 antibody 13162-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RASGRP3 Polyclonal antibody (Cat# 13162-1-AP) has 8 citations published in journals including Cancer Cell, BMC Medicine, Prostate Cancer and Prostatic Diseases, Inflammation, Metabolic Brain Diseases, American Journal of Cancer Research, Gene, and Journal of Veterinary Science. Research fields span oncology (uveal melanoma, colorectal cancer, prostate cancer, thyroid cancer), cardiovascular disease (atherosclerosis), neurodegeneration (Alzheimer's disease), and virology (infectious bronchitis virus).
| Species | Application | Title |
|---|---|---|
BMC Med Downregulation of AC092894.1 promotes oxaliplatin resistance in colorectal cancer via the USP3/AR/RASGRP3 axis
| ||
Prostate Cancer Prostatic Dis Upregulation of RASGRP3 expression in prostate cancer correlates with aggressive capabilities and predicts biochemical recurrence after radical prostatectomy. | ||
Inflammation RasGRP3 Promotes RAP1B Activity to Inhibit Endothelial Inflammation and Alleviate Atherosclerosis in High-Fat Diet-Fed ApoE(-/-) Mice. | ||
Metab Brain Dis Integrative transcriptomic and genetic analysis implicates fatty acid metabolic reprogramming in perivascular macrophages in Alzheimer's disease. | ||
Am J Cancer Res A somatic mutation of RasGRP3 decreases Na+/I- symporter expression in metastases of radioactive iodine-refractory thyroid cancer by stimulating the Akt signaling pathway. |
