RASSF3 Polyclonal antibody, PBS Only

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• RASSF3 Polyclonal antibody, PBS Only (26428-1-PBS)

• 

  WB analysis using 26428-1-AP (same clone as 26428-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 26428-1-AP (RASSF3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26428-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 26428-1-AP (same clone as 26428-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 26428-1-AP (RASSF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26428-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 26428-1-AP (same clone as 26428-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RASSF3 antibody (26428-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 26428-1-PBS in a different storage buffer formulation.

RASSF3 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 26428-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

RASSF5

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Product Information

26428-1-PBS targets RASSF3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15801

  Product name: Recombinant human RASSF3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 14-127 aa of BC100952

  Sequence: KVQMELCKPPQTSPNSGKLSPSSNGCMNTLHISSTNTVGEVIEALLKKFLVTESPAKFALYKRCHREDQVYACKLSDREHPLYLRLVAGPRTDTLSFVLREHEIGEWEAFSLPE

• Full Name: Ras association (RalGDS/AF-6) domain family member 3
• Calculated Molecular Weight: 238 aa, 28 kDa
• Observed Molecular Weight: 28-30 kDa
• GenBank Accession Number: BC100952
• Gene Symbol: RASSF3
• Gene ID (NCBI): 283349
• RRID: AB_3742188
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86WH2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RASSF3 (Ras Association Domain Family Member 3) is an emerging tumor suppressor protein and a key member of the RASSF family, characterized by a conserved Ras Association (RA) domain. RASSF3 acts as a critical node in multiple tumor-suppressive pathways. It directly stabilizes and activates the p53 protein, thereby inducing cell cycle arrest and apoptosis. RASSF3 also contributes to the maintenance of genomic integrity by participating in the DNA damage response. Furthermore, it modulates major signaling cascades such as the Hippo and NF-κB pathways, influencing cell proliferation, survival, and inflammation.