RbAp46 Polyclonal antibody

• Featured Product
• KD/KO Validated

RbAp46 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 20365-1-AP

Synonyms

RBBP7, Retinoblastoma-binding protein p46, Retinoblastoma-binding protein 7, RBBP-7, Nucleosome-remodeling factor subunit RBAP46

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Validation Data Gallery

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  WB analysis using 20365-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20365-1-AP (RbAp46 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 20365-1-AP

  IP result of anti-RbAp46 (IP:20365-1-AP, 4ug; Detection:20365-1-AP 1:4000) with A431 cells lysate 2640 ug.

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  IHC staining of human cervical cancer using 20365-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20365-1-AP (RbAp46 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 20365-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20365-1-AP (RbAp46 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 20365-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20365-1-AP (RbAp46 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20365-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20365-1-AP (RbAp46 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20365-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20365-1-AP (RbAp46 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 20365-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20365-1-AP (RbAp46 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A431 using 20365-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using RbAp46 antibody (20365-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: A431 cells, COS-7 cells, HEK-293T cells, HeLa cells
• Positive IP detected in: A431 cells
• Positive IHC detected in: human breast cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 2 publications below

Product Information

20365-1-AP targets RbAp46 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: retinoblastoma binding protein 7
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: NM_002893
• Gene Symbol: RBBP7
• Gene ID (NCBI): 5931
• RRID: AB_10697660
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBBP7, also named as RBAP46, belongs to the WD repeat RBAP46/RBAP48/MSI1 family. RBBP7 is a core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. It is a component of several complexes which regulate chromatin metabolism. The antibody is specific to RBBP7.

Publications

Species	Application	Title
human	WB	Cell Mol Gastroenterol Hepatol; Targeting HNRNPM Inhibits Cancer Stemness and Enhances Antitumor Immunity in Wnt-activated Hepatocellular Carcinoma.; Authors - Gui-Qi Zhu ; KD Validated
human	WB	Oncogene; HNF1B-mediated repression of SLUG is suppressed by EZH2 in aggressive prostate cancer.; Authors - Jianqing Wang
human	WB,IHC	Biochem Biophys Res Commun; PAFAH1B2 is a HIF1a target gene and promotes metastasis in pancreatic cancer.; Authors - Can Ma ; KD Validated
human	WB,IHC	Oncol Lett; RBBP7 is a prognostic biomarker in patients with esophageal squamous cell carcinoma.; Authors - Ning Yu ; KD Validated
human	WB	Oncogene; ZNF133 is a potent suppressor in breast carcinogenesis through dampening L1CAM, a driver for tumor progression; Authors - Lifang Li
human	WB	J Biol Chem; Elexacaftor/VX-445-mediated CFTR interactome remodeling reveals differential correction driven by mutation-specific translational dynamics; Authors - Minsoo Kim