Tested Applications
| Positive WB detected in | A431 cells, COS-7 cells, HEK-293T cells, HeLa cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human breast cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20365-1-AP targets RbAp46 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | retinoblastoma binding protein 7 |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | NM_002893 |
| Gene Symbol | RBBP7 |
| Gene ID (NCBI) | 5931 |
| RRID | AB_10697660 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16576 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RBBP7, also named as RBAP46, belongs to the WD repeat RBAP46/RBAP48/MSI1 family. RBBP7 is a core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. It is a component of several complexes which regulate chromatin metabolism. The antibody is specific to RBBP7.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RbAp46 antibody 20365-1-AP | Download protocol |
| IHC protocol for RbAp46 antibody 20365-1-AP | Download protocol |
| IP protocol for RbAp46 antibody 20365-1-AP | Download protocol |
| WB protocol for RbAp46 antibody 20365-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RbAp46 (RBBP7) polyclonal antibody (Cat# 20365-1-AP) has 11 total citations published in journals including Nucleic Acids Research, Oncogene, Journal of Biological Chemistry, International Journal of Biological Macromolecules, Cell Molecular Gastroenterology and Hepatology, Cancer Cell International, Clinical Epigenetics, Molecular Carcinogenesis, Biochemical and Biophysical Research Communications, and Oncology Letters. Associated research fields include breast cancer, prostate cancer, ovarian aging, hepatocellular carcinoma, pancreatic cancer, esophageal cancer, cystic fibrosis, and chromatin remodeling.
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res CARM1 hypermethylates the NuRD chromatin remodeling complex to promote cell cycle gene expression and breast cancer development | ||
Oncogene HNF1B-mediated repression of SLUG is suppressed by EZH2 in aggressive prostate cancer. | ||
Oncogene ZNF133 is a potent suppressor in breast carcinogenesis through dampening L1CAM, a driver for tumor progression | ||
Int J Biol Macromol Rbbp7-mediated deacetylation of Acsl4 promotes ovarian aging by enhancing ferroptosis. | ||
Cell Mol Gastroenterol Hepatol Targeting HNRNPM Inhibits Cancer Stemness and Enhances Antitumor Immunity in Wnt-activated Hepatocellular Carcinoma.
| ||
Cancer Cell Int Platelet-activating factor acetyl hydrolase IB2 dysregulated cell proliferation in ovarian cancer. |
