Tested Applications
| Positive WB detected in | mouse testis tissue, A375 cells, human lung tissue, human spleen tissue, mouse lung tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | BxPC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12230-2-AP targets RBBP9 in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2868 Product name: Recombinant human RBBP9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-186 aa of BC015938 Sequence: MASPSKAVIVPGNGGGDVTTHGWYGWVKKELEKIPGFQCLAKNMPDPITARESIWLPFMETELHCDEKTIIIGHSSGAIAAMRYAETHRVYAIVLVSAYTSDLGDENERASGYFTRPWQWEKIKANCPYIVQFGSTDDPFLPWKEQQEVADRLETKLHKFTDCGHFQNTEFHELITVVKSLLKVPA Predict reactive species |
| Full Name | retinoblastoma binding protein 9 |
| Calculated Molecular Weight | 186 aa, 21 kDa |
| Observed Molecular Weight | 22 kDa |
| GenBank Accession Number | BC015938 |
| Gene Symbol | RBBP9 |
| Gene ID (NCBI) | 10741 |
| RRID | AB_2175265 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75884 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RBBP9, also named as BOG, RBBP10, RBBP-9, RBBP-10 and Protein BOG, belongs to the RBBP9 family. It may play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-β1 through interaction with retinoblastoma and the subsequent displacement of E2F-1. RBBP9 is a tumor-associated serine hydrolase activity required for pancreatic neoplasia. It mediates suppression of TGF-β signaling is required for E-cadherin expression as loss of the serine hydrolase activity leads to a reduction in E-cadherin levels and a concomitant decrease in the integrity of tumor cell-cell junctions..RBBP9 protein levels were equivalent in paired primary tumor and nonneoplastic specimens(PMID: 20080647) The genes CyFIP2 and RbBP9, which are also miss-expressed in ALS hMSC, could serve as diagnostic biomarker tools for detection of ALS in blood samples.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for RBBP9 antibody 12230-2-AP | Download protocol |
| IHC protocol for RBBP9 antibody 12230-2-AP | Download protocol |
| IP protocol for RBBP9 antibody 12230-2-AP | Download protocol |
| WB protocol for RBBP9 antibody 12230-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RBBP9 Polyclonal antibody (Cat# 12230-2-AP) has 5 citations published in Proc Natl Acad Sci U S A, Cell Mol Gastroenterol Hepatol, Antioxid Redox Signal, Exp Hematol, and Dis Markers. Research fields include pancreatic neoplasia, intestinal inflammation and tumorigenesis, 20S proteasome regulation, human pluripotent stem cell maintenance, and ALS biomarker identification.
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A RBBP9: a tumor-associated serine hydrolase activity required for pancreatic neoplasia. | ||
Cell Mol Gastroenterol Hepatol Retinoblastoma-Binding Protein 9 Suppresses Intestinal Inflammation and Inflammation-Induced Tumorigenesis in Mice
| ||
Antioxid Redox Signal Regulation of the 20S Proteasome by a Novel Family of Inhibitory Proteins. | ||
Exp Hematol Retinoblastoma-binding proteins 4 and 9 are important for human pluripotent stem cell maintenance.
| ||
Dis Markers Two potential biomarkers identified in mesenchymal stem cells and leukocytes of patients with sporadic amyotrophic lateral sclerosis. |
