RBBP9 Monoclonal antibody

RBBP9 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1H6D8

Cat no : 66015-1-Ig

Synonyms

RBBP9

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Validation Data Gallery

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  WB analysis of A549 using 66015-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66015-1-Ig (RBBP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 66015-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66015-1-Ig (RBBP9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 66015-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66015-1-Ig (RBBP9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 66015-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas using 66015-1-Ig(RBBP9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 66015-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas using 66015-1-Ig(RBBP9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 66015-1-Ig

  Immunofluorescent analysis of Hela cells, using RBBP9 antibody 66015-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

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Tested Applications

• Positive WB detected in: A549 cells
• Positive IHC detected in: human pancreas cancer tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 66015-1-Ig is RBBP9 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: RBBP9 fusion protein Ag17864
• Full Name: retinoblastoma binding protein 9
• Calculated Molecular Weight: 186 aa, 21 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC015938
• Gene Symbol: RBBP9
• Gene ID (NCBI): 10741
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBBP9, also named as BOG, RBBP10, RBBP-9, RBBP-10 and Protein BOG, belongs to the RBBP9 family. It may play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-β1 through interaction with retinoblastoma and the subsequent displacement of E2F-1. RBBP9 is a tumor-associated serine hydrolase activity required for pancreatic neoplasia. It mediates suppression of TGF-β signaling is required for E-cadherin expression as loss of the serine hydrolase activity leads to a reduction in E-cadherin levels and a concomitant decrease in the integrity of tumor cell–cell junctions..RBBP9 protein levels were equivalent in paired primary tumor and nonneoplastic specimens(PMID:20080647)