Tested Applications
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26367-1-AP targets RBCK1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24405 Product name: Recombinant human RBCK1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 225-500 aa of BC015219 Sequence: EERARLAGEEEALRQYQQRKQQQQEGNYLQHVQLDQRSLVLNTEPAECPVCYSVLAPGEAVVLRECLHTFCRECLQGTIRNSQEAEVSCPFIDNTYSCSGKLLEREIKALLTPEDYQRFLDLGISIAENRSAFSYHCKTPDCKGWCFFEDDVNEFTCPVCFHVNCLLCKAIHEQMNCKEYQEDLALRAQNDVAARQTTEMLKVMLQQGEAMRCPQCQIVVQKKDGCDWIRCTVCHTEICWVTKGPRWGPGGPGDTSGGCRCRVNGIPCHPSCQNCH Predict reactive species |
| Full Name | RanBP-type and C3HC4-type zinc finger containing 1 |
| Calculated Molecular Weight | 58 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC015219 |
| Gene Symbol | RBCK1 |
| Gene ID (NCBI) | 10616 |
| RRID | AB_2880493 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BYM8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RBCK1 antibody 26367-1-AP | Download protocol |
| IP protocol for RBCK1 antibody 26367-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-RBCK1 (HOIL-1L) antibody, catalog number 26367-1-AP, has 12 total citations. Citations appear in journals including Cell Death & Disease, Cell Communication & Signaling, Oncogene, EMBO Journal, Frontiers in Immunology, Journal of Clinical Immunology, Human Cell, BMC Musculoskeletal Disorders, Discover Oncology, Biochemical and Biophysical Research Communications, and Archives of Oral Biology. Research fields span renal cell carcinoma, breast cancer, ovarian cancer, ubiquitination, NF-κB signaling, autoinflammation, PANoptosis, and protein–protein interactions.
| Species | Application | Title |
|---|---|---|
Cell Death Dis RBCK1 regulates the progression of ER-positive breast cancer through the HIF1α signaling | ||
Cell Death Dis RBCK1 promotes p53 degradation via ubiquitination in renal cell carcinoma.
| ||
Cell Commun Signal RBCK1 is an endogenous inhibitor for triple negative breast cancer via hippo/YAP axis
| ||
Oncogene The E3 ligase RBCK1 reduces the sensitivity of ccRCC to sunitinib through the ANKRD35-MITD1-ANXA1 axis
| ||
EMBO J STING induces HOIP-mediated synthesis of M1 ubiquitin chains to stimulate NF-κB signaling | ||
Front Immunol Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma |
