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RBM10 Polyclonal antibody
RBM10 Polyclonal Antibody for IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, RIP, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 14423-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human brain tissue |
Positive IP detected in | mouse brain tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 4 publications below |
WB | See 5 publications below |
IHC | See 2 publications below |
IP | See 1 publications below |
RIP | See 1 publications below |
Product Information
14423-1-AP targets RBM10 in WB, RIP, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RBM10 fusion protein Ag5788 |
Full Name | RNA binding motif protein 10 |
Calculated molecular weight | 104 kDa |
Observed molecular weight | 115 kDa |
GenBank accession number | BC024153 |
Gene symbol | RBM10 |
Gene ID (NCBI) | 8241 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RBM10, also named as RNA-binding protein 10, is a 930 amino acid protein, which is in the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. RBM10 may be involved in post-transcriptional processing, most probably in mRNA splicing. RBM10 has some isoforms with MV 130 and 115 kDa (PMID: 25889998 and PMID: 18315527) .
Protocols
Product Specific Protocols | |
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WB protocol for RBM10 antibody 14423-1-AP | Download protocol |
IP protocol for RBM10 antibody 14423-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Oncogene RNA-binding motif protein 10 induces apoptosis and suppresses proliferation by activating p53.
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BMC Mol Biol Splicing arrays reveal novel RBM10 targets, including SMN2 pre-mRNA.
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J Cell Mol Med RBM10 inhibits cell proliferation of lung adenocarcinoma via RAP1/AKT/CREB signalling pathway. | ||
Cancer Cell Int RBM10 regulates alternative splicing of lncRNA Neat1 to inhibit the invasion and metastasis of NSCLC
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Life Sci RBM10 recruits METTL3 to induce N6-methyladenosine-MALAT1-dependent modification, inhibiting the invasion and migration of NSCLC
|