RBM10 Polyclonal antibody

• Featured Product
• KD/KO Validated

RBM10 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 14423-1-AP

Synonyms

DXS8237E, GPATC9, GPATCH9, KIAA0122, RBM10, RNA binding motif protein 10, RNA binding protein 10, RNA binding protein S1 1, S1 1, ZRANB5

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Validation Data Gallery

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  WB analysis of human brain using 14423-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14423-1-AP (RBM10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14423-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14423-1-AP (RBM10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14423-1-AP

  IP result of anti-RBM10 (IP:14423-1-AP, 4ug; Detection:14423-1-AP 1:800) with mouse brain tissue lysate 4000ug.

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  IF Staining of mouse brain using 14423-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RBM10 antibody (14423-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14423-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RBM10 antibody (14423-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14423-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RBM10 antibody (14423-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IF detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Product Information

14423-1-AP targets RBM10 in WB, RIP, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBM10 fusion protein Ag5788
• Full Name: RNA binding motif protein 10
• Calculated Molecular Weight: 104 kDa
• Observed Molecular Weight: 115 kDa
• GenBank Accession Number: BC024153
• Gene Symbol: RBM10
• Gene ID (NCBI): 8241
• RRID: AB_2175593
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM10, also named as RNA-binding protein 10, is a 930 amino acid protein, which is in the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. RBM10 may be involved in post-transcriptional processing, most probably in mRNA splicing. RBM10 has some isoforms with MV 130 and 115 kDa (PMID: 25889998 and PMID: 18315527) .

Publications

Species	Application	Title
human	WB,IHC,IF,IP	Proc Natl Acad Sci U S A; RNA-binding motif protein 10 inactivates c-Myc by partnering with ribosomal proteins uL18 and uL5; Authors - Hyemin Lee ; KD Validated
human	WB	Oncogene; RNA-binding motif protein 10 induces apoptosis and suppresses proliferation by activating p53.; Authors - Ji Hoon Jung ; KD Validated
human	WB	BMC Mol Biol; Splicing arrays reveal novel RBM10 targets, including SMN2 pre-mRNA.; Authors - Leslie C Sutherland ; KD Validated
human	WB	J Cell Mol Med; RBM10 inhibits cell proliferation of lung adenocarcinoma via RAP1/AKT/CREB signalling pathway.; Authors - Xin Jin
human	WB,CoIP	Exp Cell Res; RBM10 regulates the tumorigenic potential of human cancer cells by modulating PPM1B and YBX1 activities; Authors - Yueyang Li ; KD Validated
human	WB,IHC,RIP	Cancer Cell Int; RBM10 regulates alternative splicing of lncRNA Neat1 to inhibit the invasion and metastasis of NSCLC; Authors - Shan Cong ; KD Validated