RBM10 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• RBM10 Polyclonal antibody (14423-1-AP)

• Featured Product
• KD/KO Validated

RBM10 Polyclonal Antibody for WB, IF-P, IP, ELISA

Cat No. 14423-1-AP Copied!

Publications (14)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, IP, CoIP, RIP, ELISA

Synonyms

DXS8237E, G patch domain-containing protein 9, GPATC9, GPATCH9, KIAA0122

• 

  WB analysis using 14423-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14423-1-AP (RBM10 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 14423-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14423-1-AP (RBM10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 14423-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14423-1-AP (RBM10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 14423-1-AP

  IP result of anti-RBM10 (IP:14423-1-AP, 4ug; Detection:14423-1-AP 1:800) with mouse brain tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse brain using 14423-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RBM10 antibody (14423-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse brain using 14423-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RBM10 antibody (14423-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse brain using 14423-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RBM10 antibody (14423-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for RBM10 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: A549 cells, human brain tissue, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14423-1-AP targets RBM10 in WB, IHC, IF-P, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5788

  Product name: Recombinant human RBM10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 601-930 aa of BC024153

  Sequence: QSQQYLYWDGERRTYVPALEQSADGHKETGAPSKEGKEKKEKHKTKTAQQIAKDMERWARSLNKQKENFKNSFQPISSLRDDERRESATADAGYAILEKKGALAERQHTSMDLPKLASDDRPSPPRGLVAAYSGESDSEEEQERGGPEREEKLTDWQKLACLLCRRQFPSKEALIRHQQLSGLHKQNLEIHRRAHLSENELEALEKNDMEQMKYRDRAAERREKYGIPEPPEPKRRKYGGISTASVDFEQPTRDGLGSDNIGSRMLQAMGWKEGSGLGRKKQGIVTPIEAQTRVRGSGLGARGSSYGVTSTESYKETLHKTMVTRFNEAQ

• Full Name: RNA binding motif protein 10
• Calculated Molecular Weight: 104 kDa
• Observed Molecular Weight: 115 kDa
• GenBank Accession Number: BC024153
• Gene Symbol: RBM10
• Gene ID (NCBI): 8241
• RRID: AB_2175593
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P98175
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM10, also named as RNA-binding protein 10, is a 930 amino acid protein, which is in the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. RBM10 may be involved in post-transcriptional processing, most probably in mRNA splicing. RBM10 has some isoforms with MV 100-130 kDa (PMID: 25889998 and PMID: 18315527) .

Publications

What published studies show

The Rabbit anti-RBM10 (Affinity Purified) antibody (Cat# 14423-1-AP) has 14 total citations. Citations appear in journals including PNAS, Oncogene, EMBO Journal, Scientific Reports, Life Sciences, Cancer Cell International, mSystems, PLoS Pathogens, Journal of Cellular and Molecular Medicine, Experimental Cell Research, Veterinary Microbiology, BMC Molecular Biology, Oxidative Medicine and Cellular Longevity, and American Journal of Translational Research. Research fields span RNA splicing regulation, cancer biology (NSCLC, colorectal, endometrial, pan-cancer), virology (HIV-1, cardiovirus, PEDV), and epigenetic modifications.

Automatically generated from the 14 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IP,IHC,IF	Proc Natl Acad Sci U S A; RNA-binding motif protein 10 inactivates c-Myc by partnering with ribosomal proteins uL18 and uL5; Authors - Hyemin Lee ; KD Validated
human	WB	Oncogene; RNA-binding motif protein 10 induces apoptosis and suppresses proliferation by activating p53.; Authors - Ji Hoon Jung ; KD Validated
human	WB	EMBO J; A transcription-independent mechanism determines rapid periodic fluctuations of BRCA1 expression; Authors - Chen Nie
human	WB	Oxid Med Cell Longev; RBM10 Is a Biomarker Associated with Pan-Cancer Prognosis and Immune Infiltration: System Analysis Combined with In Vitro and Vivo Experiments; Authors - Yingyue Cao ; KD Validated
human	WB,RIP,IHC	Cancer Cell Int; RBM10 regulates alternative splicing of lncRNA Neat1 to inhibit the invasion and metastasis of NSCLC; Authors - Shan Cong ; KD Validated
human	WB,IP,IHC	Life Sci; RBM10 recruits METTL3 to induce N6-methyladenosine-MALAT1-dependent modification, inhibiting the invasion and migration of NSCLC; Authors - Yingshu Cao ; KD Validated