RBM10 Polyclonal antibody

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  WB analysis using 14423-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14423-1-AP (RBM10 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14423-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14423-1-AP (RBM10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14423-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14423-1-AP (RBM10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14423-1-AP

  IP result of anti-RBM10 (IP:14423-1-AP, 4ug; Detection:14423-1-AP 1:800) with mouse brain tissue lysate 4000ug.

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  IF Staining of mouse brain using 14423-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RBM10 antibody (14423-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 14423-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RBM10 antibody (14423-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14423-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RBM10 antibody (14423-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

RBM10 Polyclonal Antibody for WB, IF-P, IP, ELISA

Cat No. 14423-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, CoIP, RIP, ELISA

Synonyms

DXS8237E, G patch domain-containing protein 9, GPATC9, GPATCH9, KIAA0122

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Tested Applications

• Positive WB detected in: A549 cells, human brain tissue, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Product Information

14423-1-AP targets RBM10 in WB, IHC, IF-P, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5788

  Product name: Recombinant human RBM10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 601-930 aa of BC024153

  Sequence: QSQQYLYWDGERRTYVPALEQSADGHKETGAPSKEGKEKKEKHKTKTAQQIAKDMERWARSLNKQKENFKNSFQPISSLRDDERRESATADAGYAILEKKGALAERQHTSMDLPKLASDDRPSPPRGLVAAYSGESDSEEEQERGGPEREEKLTDWQKLACLLCRRQFPSKEALIRHQQLSGLHKQNLEIHRRAHLSENELEALEKNDMEQMKYRDRAAERREKYGIPEPPEPKRRKYGGISTASVDFEQPTRDGLGSDNIGSRMLQAMGWKEGSGLGRKKQGIVTPIEAQTRVRGSGLGARGSSYGVTSTESYKETLHKTMVTRFNEAQ

• Full Name: RNA binding motif protein 10
• Calculated Molecular Weight: 104 kDa
• Observed Molecular Weight: 115 kDa
• GenBank Accession Number: BC024153
• Gene Symbol: RBM10
• Gene ID (NCBI): 8241
• RRID: AB_2175593
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P98175
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM10, also named as RNA-binding protein 10, is a 930 amino acid protein, which is in the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. RBM10 may be involved in post-transcriptional processing, most probably in mRNA splicing. RBM10 has some isoforms with MV 100-130 kDa (PMID: 25889998 and PMID: 18315527) .

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IP,IHC,IF	Proc Natl Acad Sci U S A; RNA-binding motif protein 10 inactivates c-Myc by partnering with ribosomal proteins uL18 and uL5; Authors - Hyemin Lee ; KD Validated
human	WB	Oncogene; RNA-binding motif protein 10 induces apoptosis and suppresses proliferation by activating p53.; Authors - Ji Hoon Jung ; KD Validated
human	WB,CoIP	Exp Cell Res; RBM10 regulates the tumorigenic potential of human cancer cells by modulating PPM1B and YBX1 activities; Authors - Yueyang Li ; KD Validated
human	WB	BMC Mol Biol; Splicing arrays reveal novel RBM10 targets, including SMN2 pre-mRNA.; Authors - Leslie C Sutherland ; KD Validated
human	WB,IHC	Sci Rep; RBM10 suppresses colorectal cancer invasion by regulating LncRNA SNHG17 alternative splicing; Authors - Linxia An
human	WB	EMBO J; A transcription-independent mechanism determines rapid periodic fluctuations of BRCA1 expression; Authors - Chen Nie