|Positive WB detected in||human brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IF detected in||mouse brain tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14423-1-AP targets RBM10 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RBM10 fusion protein Ag5788|
|Full Name||RNA binding motif protein 10|
|Calculated molecular weight||104 kDa|
|Observed molecular weight||115 kDa|
|GenBank accession number||BC024153|
|Gene ID (NCBI)||8241|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
RBM10, also named as RNA-binding protein 10, is a 930 amino acid protein, which is in the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. RBM10 may be involved in post-transcriptional processing, most probably in mRNA splicing. RBM10 has some isoforms with MV 130 and 115 kDa (PMID: 25889998 and PMID: 18315527) .
RNA-binding motif protein 10 induces apoptosis and suppresses proliferation by activating p53.
BMC Mol Biol
Splicing arrays reveal novel RBM10 targets, including SMN2 pre-mRNA.
J Cell Mol Med
RBM10 inhibits cell proliferation of lung adenocarcinoma via RAP1/AKT/CREB signalling pathway.
Cancer Cell Int
RBM10 regulates alternative splicing of lncRNA Neat1 to inhibit the invasion and metastasis of NSCLC
RBM10 recruits METTL3 to induce N6-methyladenosine-MALAT1-dependent modification, inhibiting the invasion and migration of NSCLC
A transcription-independent mechanism determines rapid periodic fluctuations of BRCA1 expression