RBM10 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84104-4-RR (same clone as 84104-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84104-4-RR (RBM10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84104-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84104-4-RR (same clone as 84104-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84104-4-RR (RBM10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84104-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84104-4-RR (same clone as 84104-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84104-4-RR (RBM10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84104-4-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84104-4-RR (same clone as 84104-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84104-4-RR (RBM10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84104-4-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84104-4-RR (same clone as 84104-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84104-4-RR (RBM10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84104-4-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 84104-4-RR (same clone as 84104-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using RBM10 antibody (84104-4-RR, Clone: 241151G11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84104-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84104-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84104-4-RR against Human RBM10 were performed. The affinity constant is 0.33 nM.

RBM10 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 84104-4-PBS

Clone No.241151G11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

KIAA0122, GPATCH9, GPATC9, G patch domain-containing protein 9, DXS8237E

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Product Information

84104-4-PBS targets RBM10 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5788

  Product name: Recombinant human RBM10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 601-930 aa of BC024153

  Sequence: QSQQYLYWDGERRTYVPALEQSADGHKETGAPSKEGKEKKEKHKTKTAQQIAKDMERWARSLNKQKENFKNSFQPISSLRDDERRESATADAGYAILEKKGALAERQHTSMDLPKLASDDRPSPPRGLVAAYSGESDSEEEQERGGPEREEKLTDWQKLACLLCRRQFPSKEALIRHQQLSGLHKQNLEIHRRAHLSENELEALEKNDMEQMKYRDRAAERREKYGIPEPPEPKRRKYGGISTASVDFEQPTRDGLGSDNIGSRMLQAMGWKEGSGLGRKKQGIVTPIEAQTRVRGSGLGARGSSYGVTSTESYKETLHKTMVTRFNEAQ

• Full Name: RNA binding motif protein 10
• Calculated Molecular Weight: 104 kDa
• Observed Molecular Weight: 100-120 kDa
• GenBank Accession Number: BC024153
• Gene Symbol: RBM10
• Gene ID (NCBI): 8241
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P98175
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RBM10, also named as RNA-binding protein 10, is a 930 amino acid protein, which is in the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. RBM10 may be involved in post-transcriptional processing, most probably in mRNA splicing. RBM10 has some isoforms with MV 100-130 kDa (PMID: 25889998 and PMID: 18315527) .