Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, NIH/3T3 cells, L02 cells, HT-1080 cells, THP-1 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human testis tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18893-1-AP targets RBM16 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13499 Product name: Recombinant human RBM16 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 921-1271 aa of BC070071 Sequence: MPMLDIRPGLIPQAPGPRFPLIQPGIPPQRGIPPPSVLDSALHPPPRGPFPPGDIFSQPERPFLAPGRQSVDNVTNPEKRIPLGNDNIQQEGDRDYRFPPIETRESISRPPPVDVRDVVGRPIDPREGPGRPPLDGRDHFGRPPVDIRENLVRPGIDHLGRRDHFGFNPEKPWGHRGDFDEREHRVLPVYGGPKGLHEERGRFRSGNYRFDPRSGPWNRGFGQEVHRDFDDRRRPWERQRDRDDRDFDFCREMNGNRLGRDRIQNTWVPPPHARVFDYFEGATSQRKGDNVPQVNGENTERHAQPPPIPVQNDPELYEKLTSSNEINKEKSDTVADIESEPVVESTETEGT Predict reactive species |
| Full Name | RNA binding motif protein 16 |
| Calculated Molecular Weight | 1271 aa, 141 kDa |
| Observed Molecular Weight | 170 kDa |
| GenBank Accession Number | BC070071 |
| Gene Symbol | RBM16 |
| Gene ID (NCBI) | 22828 |
| RRID | AB_2175908 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UPN6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RBM16, also named as SCAF8, CCAP7, KIAA1116, is a 1271 amino acid protein, which interacts with POLR2A. RBM16 may play a role in mRNA processing. Immunoblot analysis of the total rat liver nuclear and nuclear matrix protein fractions revealed a single band with an apparent molecular mass of 170 kDa. While the apparent molecular mass is slightly larger than the 140 kDa predicted from the cDNA sequence, we note that the protein is rich in proline residues (10%) and therefore may display aberrant mobility in SDS gel electrophoresis. (PMID: 9528809 )
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RBM16 antibody 18893-1-AP | Download protocol |
| IHC protocol for RBM16 antibody 18893-1-AP | Download protocol |
| IP protocol for RBM16 antibody 18893-1-AP | Download protocol |
| WB protocol for RBM16 antibody 18893-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RBM16 Polyclonal antibody (Cat# 18893-1-AP) has 1 total citation. The cited publication appeared in Cell Mol Biol Lett (2026), investigating how PRKN mediates ubiquitination of SCAF8 (RBM16) to reduce mRNA stability of KLF5 and its transcriptional activation of EFNA3 in colorectal cancer. The research field is cancer biology, specifically colorectal cancer molecular mechanisms involving RNA processing and transcriptional regulation.
| Species | Application | Title |
|---|---|---|
Cell Mol Biol Lett PRKN mediates the ubiquitination of SCAF8 to reduce the mRNA stability of KLF5 and its transcriptional activation of EFNA3 in colorectal cancer. |
