RBM20 Polyclonal antibody

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  IHC staining of rat heart using 31177-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 31177-1-AP (RBM20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 31177-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31177-1-AP (RBM20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RBM20 Polyclonal Antibody for IHC, ELISA

Cat No. 31177-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Synonyms

RNA binding motif protein 20, RNA binding protein

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Tested Applications

• Positive IHC detected in: rat heart tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31177-1-AP targets RBM20 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag34322

  Product name: Recombinant human RBM20 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 667-840 aa of NM_001134363

  Sequence: ADWGNGRDSWEHSPYARREEERDPAPWRDNGDDKRDRMDPWAHDRKHHPRQLDKAELDERPEGGRPHREKYPRSGSPNLPHSVSSYKSREDGYYRKEPKAKSDKYLKQQQDAPGRSRRKDEARLRESRHPHPDDSGKEDGLGPKVTRAPEGAKAKQNEKNKTKRTDRDQEGADD

• Full Name: RNA binding motif protein 20
• Calculated Molecular Weight: 134 kDa
• GenBank Accession Number: NM_001134363
• Gene Symbol: RBM20
• Gene ID (NCBI): 282996
• RRID: AB_3669884
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q5T481
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM20 (RNA binding motif protein 20) is a striated muscle-specific splicing factor that regulates alternative splicing of many cardiac genes encoding sarcomere and calcium-regulatory proteins (PMID: 36417486). RBM20 has been identified as a crucial RNA-binding protein that controls the splicing of TTN (PMID: 30547036). RBM20 is expressed in all striated muscles but is highest in cardiac tissue (PMID: 34575212).