RBM22 Polyclonal antibody, PBS Only

• Home
• Products
• Primary Antibodies
• RBM22 Polyclonal antibody, PBS Only (22103-1-PBS)

• 

  WB analysis of Raji using 22103-1-AP (same clone as 22103-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 22103-1-AP (RBM22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22103-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 22103-1-AP (same clone as 22103-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22103-1-AP (RBM22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22103-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 22103-1-AP (same clone as 22103-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22103-1-AP (RBM22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22103-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat stomach using 22103-1-AP (same clone as 22103-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 22103-1-AP (RBM22 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22103-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 22103-1-AP (same clone as 22103-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RBM22 antibody (22103-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 22103-1-PBS in a different storage buffer formulation.

RBM22 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 22103-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

199G4, fSAP47, Pre mRNA splicing factor RBM22, Pre-mRNA-splicing factor RBM22, RNA binding motif protein 22

---

Product Information

22103-1-PBS targets RBM22 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17315

  Product name: Recombinant human RBM22 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-420 aa of BC003402

  Sequence: MATSLGSNTYNRQNWEDADFPILCQTCLGENPYIRMTKEKYGKECKICARPFTVFRWCPGVRMRFKKTEVCQTCSKLKNVCQTCLLDLEYGLPIQVRDAGLSFKDDMPKSDVNKEYYTQNMEREISNSDGTRPVGMLGKATSTSDMLLKLARTTPYYKRNRPHICSFWVKGECKRGEECPYRHEKPTDPDDPLADQNIKDRYYGINDPVADKLLKRASTMPRLDPPEDKTITTLYVGGLGDTITETDLRNHFYQFGEIRTITVVQRQQCAFIQFATRQAAEVAAEKSFNKLIVNGRRLNVKWGRSQAARGKEKEKDGTTDSGIKLEPVPGLPGALPPPPAAEEEASANYFNLPPSGPPAVVNIALPPPPGIAPPPPPGFGPHMFHPMGPPPPFMRAPGPIHYPSQDPQRMGAHAGKHSSP

• Full Name: RNA binding motif protein 22
• Calculated Molecular Weight: 420 aa, 47 kDa
• Observed Molecular Weight: 47-50 kDa
• GenBank Accession Number: BC003402
• Gene Symbol: RBM22
• Gene ID (NCBI): 55696
• RRID: AB_2878996
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NW64
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RBM22 is mainly involved in pre-mRNA splicing, playing the essential role of maintaining the conformation of the catalytic core of the spliceosome and acting as a bridge between the catalytic core and other essential protein components of the spliceosome. RBM22 is also involved in gene regulation, and is able to bind DNA, acting as a bona fide transcription factor on a large number of target genes. Due to its wide scope in the regulation of gene expression, RBM22 has been associated with several pathologies and, notably, with the aggressiveness of cancer cells and with the phenotype of a myelodysplastic syndrome. (PMID: 35158909)