RBM26 Polyclonal antibody

RBM26 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16823-1-AP

Synonyms

ARRS2, C13orf10, CTCL tumor antigen se70 2, PRO1777, RBM26, RNA binding motif protein 26, RNA binding protein 26, RP11 255E21.1, SE70 2, ZC3H17

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Validation Data Gallery

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  WB analysis using 16823-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16823-1-AP (RBM26 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 16823-1-AP

  IP result of anti-RBM26 (IP:16823-1-AP, 4ug; Detection:16823-1-AP 1:5000) with HEK-293 cells lysate 1400 ug.

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  IHC staining of human brain using 16823-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16823-1-AP (RBM26 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 16823-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16823-1-AP (RBM26 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 16823-1-AP

  Immunofluorescent analysis of HepG2 cells, using RBM26 antibody 16823-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16823-1-AP targets RBM26 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBM26 fusion protein Ag10398
• Full Name: RNA binding motif protein 26
• Calculated Molecular Weight: 983 aa, 111 kDa
• Observed Molecular Weight: 125 kDa
• GenBank Accession Number: BC000791
• Gene Symbol: RBM26
• Gene ID (NCBI): 64062
• RRID: AB_2175942
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.