RBM27 Polyclonal antibody

• 

  WB analysis of HeLa using 31850-1-AP

  WB result of RBM27 antibody (31850-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM27 transfected HeLa cells.

• 

  WB analysis using 31850-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31850-1-AP (RBM27 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of U2OS using 31850-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RBM27 antibody (31850-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

RBM27 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 31850-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

KIAA1311, RNA Binding Motif Protein 27, RNA-Binding Protein 27

---

Tested Applications

• Positive WB detected in: HeLa cells, Hela cell, jurkat cell, K-562 cell
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31850-1-AP targets RBM27 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag35822

  Product name: Recombinant human RBM27 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 680-815 aa of NM_018989

  Sequence: TLQSSAQLLLQQQQTLSHLSQQHHHLPQHLHQQQVLVAQSAPSTVHGGIQKMMSKPQTSGAYVLNKVPVKHRLGHAGGNQSDASHLLNQSGGAGEDCQIFSTPGHPKMIYSSSNLKTPSKLCSGSKSHDVQEVLKK

• Full Name: RNA binding motif protein 27
• Calculated Molecular Weight: 119 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: NM_018989
• Gene Symbol: RBM27
• Gene ID (NCBI): 54439
• RRID: AB_3670129
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9P2N5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM27 has been identified as a candidate autism gene, and is present in cytoplasmic and and nuclear speckles. RBM27 encodes a protein containing an RNA-Binding Motif, which confers its ability to bind RNA. Therefore, RBM27 is important in mRNA processing and the turnover of nuclear polyadenylate (pA+) RNA.