RBM28 Polyclonal antibody

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• RBM28 Polyclonal antibody (16484-1-AP)

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RBM28 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 16484-1-AP Copied!

Publications (4)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, RIP, ELISA

Synonyms

RNA-binding protein 28, RNA-binding motif protein 28, RNA binding protein 28, RNA binding motif protein 28

• 

  WB analysis of NIH/3T3 using 16484-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16484-1-AP (RBM28 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of U-251 using 16484-1-AP

  WB result of RBM28 antibody (16484-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM28 transfected U-251 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of NIH/3T3 using 16484-1-AP

  IP result of anti-RBM28 (IP:16484-1-AP, 4ug; Detection:16484-1-AP 1:1000) with NIH/3T3 cells lysate 1120 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of NIH/3T3 using 16484-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 16484-1-AP (RBM28 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, U-251 cells
• Positive IP detected in: NIH/3T3 cells
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16484-1-AP targets RBM28 in WB, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9606

  Product name: Recombinant human RBM28 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 411-760 aa of BC013889

  Sequence: QLKVDLAVTRDEAAKLQTTKVKKPTGTRNLYLAREGLIRAGTKAAEGVSAADMAKRERFELLKHQKLKDQNIFVSRTRLCLHNLPKAVDDKQLRKLLLSATSGEKGVRIKECRVMRDLKGVHGNMKGQSLGYAFAEFQEHEHALKALRLINNNPEIFGPLKRPIVEFSLEDRRKLKMKELRIQRSLQKMRSKPATGEPQKGQPEPAKDQQQKAAQHHTEEQSKVPPEQKRKAGSTSWTGFQTKAEVEQVELPDGKKRRKVLALPSHRGPKIRLRDKGKVKPVHPKKPKPQINQWKQEKQQLSSEQVSRKKAKGNKTETRFNQLVEQYKQKLLGPSKGAPLAKRSKWFDS

• Full Name: RNA binding motif protein 28
• Calculated Molecular Weight: 756 aa, 86 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC013889
• Gene Symbol: RBM28
• Gene ID (NCBI): 55131
• RRID: AB_2238116
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NW13
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The anti-RBM28 antibody (Cat# 16484-1-AP) has a total of 4 citations appearing in Science Advances, Cancer Letters, Journal of Biological Chemistry, and Nature Communications. The associated research fields span oncology (chronic myeloid leukemia, hepatocellular carcinoma), RNA biology and splicing regulation, p53 transcriptional control, and mutant TP53-driven suppression of antitumor immunity.

Automatically generated from the 4 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Sci Adv; Transcriptional readthrough precedes alternative splicing programs triggered in CML cells by imatinib.; Authors - Paulina Podszywałow-Bartnicka
human	WB	Cancer Lett; RNA-binding motif protein 28 enhances angiogenesis by improving STAT3 translation in hepatocellular carcinoma; Authors - Hexu Han
human	WB,IF	J Biol Chem; RNA binding protein RBM28 can translocate from the nucleolus to the nucleoplasm to inhibit the transcriptional activity of p53.; Authors - Xin Lin
human, mouse	WB,RIP,IP,IF	Nat Commun; Mutant TP53 hijacks RNA-splicing factor RBM28 to suppress double-stranded RNA triggered antitumor immunity.; Authors - Tao Xiang ; KO Validated