Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32834-1-AP targets RBM34 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37711 Product name: Recombinant human RBM34 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 77-425 aa of BC029451 Sequence: TKRNEEEESTSQIERPLSQEPAKKVKAKKKHTNAEKKLADRESALASADLEEEIHQKQGQKRKNSQPGVKVADRKILDDTEDTVVSQRKKIQINQEEERLKNERTVFVGNLPVTCNKKKLKSFFKEYGQIESVRFRSLIPAEGTLSKKLAAIKRKIHPDQKNINAYVVFKEESAATQALKRNGAQIADGFRIRVDLASETSSRDKRSVFVGNLPYKVEESAIEKHFLDCGSIMAVRIVRDKMTGIGKGFGYVLFENTDSVHLALKLNNSELMGRKLRVMRSVNKEKFKQQNSNPRLKNVSKPKQGLNFTSKTAEGHPKSLFIGEKAVLLKTKKKGQKKSGRPKKQRKQK Predict reactive species |
| Full Name | RNA binding motif protein 34 |
| Calculated Molecular Weight | 430 aa, 49 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC029451 |
| Gene Symbol | RBM34 |
| Gene ID (NCBI) | 23029 |
| RRID | AB_3742756 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P42696 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RBM34 (RNA Binding Motif Protein 34) belongs to the RNA-binding motif protein (RBM) family, whose members all contain an RNA recognition motif (RRM) that enables specific binding to RNA molecules and participation in multiple aspects of RNA metabolism. RBM34 is primarily localized in the nucleus and functionally acts as an RNA-binding protein that plays an important role in pre-mRNA splicing regulation-it modulates alternative splicing events through interactions with splicing factors, thereby influencing mRNA maturation and diversity.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RBM34 antibody 32834-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |

