RBM4 Polyclonal antibody

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  WB analysis of HeLa using 11614-1-AP

  WB result of RBM4 antibody (11614-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM4 transfected HeLa cells.

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  WB analysis of mouse brain using 11614-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11614-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11614-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11614-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11614-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11614-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 11614-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11614-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 11614-1-AP

  IP result of anti-RBM4 (IP:11614-1-AP, 3ug; Detection:11614-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

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  IHC staining of human gliomas using 11614-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11614-1-AP (RBM4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 11614-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11614-1-AP (RBM4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 11614-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11614-1-AP (RBM4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11614-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11614-1-AP (RBM4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 11614-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11614-1-AP (RBM4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

RBM4 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 11614-1-AP

Publications(21)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, RIP, ELISA, PLA

Synonyms

hLark, LARK, Lark homolog, RBM4, RBM4A, RNA binding motif protein 4, RNA binding motif protein 4a, RNA binding protein 4, ZCCHC21, ZCRB3A

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, human heart tissue, human kidney tissue, HeLa cells
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human gliomas tissue, human lung cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 18 publications below
• IHC: See 7 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• RIP: See 1 publications below

Product Information

11614-1-AP targets RBM4 in WB, IHC, IF, IP, RIP, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2190

  Product name: Recombinant human RBM4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-177 aa of BC021120

  Sequence: MVKLFIGNLPREATEQEIRSLFEQYGKVLECDIIKNYGFVHIEDKTAAEDAIRNLHHYKLHGVNINVEASKNKSKTSTKLHVGNISPTCTNKELRAKFEEYGPVIECDIVKDYAFVHMERAEDAVEAIRGLDNTEFQGEPPSLGRGLNTRLCAENGWISKRRGLVKITAVGWLVMKK

• Full Name: RNA binding motif protein 4
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC021120
• Gene Symbol: RBM4
• Gene ID (NCBI): 5936
• RRID: AB_2176095
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BWF3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM4, RNA-binding motif protein4, contains 2 RRM-type RNA-binding motif and a retroviral-type(RT) zinc finger. RNA-binding factor participates in number of aspects of cellular processes such as alternative splicing of pre-mRNA and translation regulation. RBM4 recruits eIF4A1 to stimulate IRES-dependent translation in responds to cellular stress. Once assembled at the rHRE, the HIF2A-RBM4-eIF4E2 complex captures the 5-prime cap and targets mRNAs to polysomes for active translation, thereby evading hypoxia-induced repression of protein synthesis. This is a rabbit polyclonal antibody raised against the full-length of human RBM4

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nature; An oxygen-regulated switch in the protein synthesis machinery.; Authors - Uniacke James J
human	WB, IHC	Cancer Cell; The splicing factor RBM4 controls apoptosis, proliferation, and migration to suppress tumor progression.; Authors - Yang Wang ; KD Validated
human	WB	Nucleic Acids Res; Modulation of alternative splicing during early infection of human primary B lymphocytes with Epstein-Barr virus (EBV): a novel function for the viral EBNA-LP protein.; Authors - Evelyne Manet
human	WB	Nat Commun; A splicing isoform of TEAD4 attenuates the Hippo-YAP signalling to inhibit tumour proliferation.; Authors - Yangfan Qi
human	WB	Int J Mol Sci; G-Quadruplex Regulation of VEGFA mRNA Translation by RBM4.; Authors - Kangkang Niu ; KD Validated
human	IHC	Sci Rep; Prognostic value of decreased expression of RBM4 in human gastric cancer.; Authors - Hongmei Yong