RBM46 Polyclonal antibody

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• RBM46 Polyclonal antibody (24368-1-AP)

RBM46 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 24368-1-AP Copied!

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Cancer/testis antigen 68, CT68, Probable RNA-binding protein 46, RNA-binding motif protein 46

• 

  WB analysis using 24368-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24368-1-AP (RBM46 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 24368-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24368-1-AP (RBM46 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 24368-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24368-1-AP (RBM46 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 24368-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24368-1-AP (RBM46 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat testis using 24368-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 24368-1-AP (RBM46 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of Jurkat using 24368-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using RBM46 antibody (24368-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Jurkat cells, LNCap cells, PC-3 cells, THP-1 cells
• Positive IHC detected in: human kidney tissue, mouse testis tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24368-1-AP targets RBM46 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21653

  Product name: Recombinant human RBM46 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 316-533 aa of BC028588

  Sequence: RQHLNGQISPNSENLIVFANKEESHPKTLGKLPTLPARLNGQHSPSPPEVERCTYPFYPGTKLTPISMYSLKSNHFNSAVMHLDYYCNKNNWAPPEYYLYSTTSQDGKVLLVYKIVIPAIANGSQSYFMPDKLCTTLEDAKELAAQFTLLHLDYNFHRSSINSLSPVSATLSSGTPSVLPYTSRPYSYPGYPLSPTISLANGSHVGQRLCISNQASFF

• Full Name: RNA binding motif protein 46
• Calculated Molecular Weight: 533 aa, 60 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC028588
• Gene Symbol: RBM46
• Gene ID (NCBI): 166863
• RRID: AB_2879509
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TBY0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.