RBM47 Polyclonal antibody

• 

  WB analysis of A549 using 23902-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 23902-1-AP (RBM47 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 23902-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 23902-1-AP (RBM47 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

RBM47 Polyclonal Antibody for WB, ELISA

Cat No. 23902-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IP, ELISA

Synonyms

NET18, RBM47, RNA binding motif protein 47, RNA binding protein 47

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Tested Applications

• Positive WB detected in: A549 cells, rat lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IP: See 1 publications below

Product Information

23902-1-AP targets RBM47 in WB, IP, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20984

  Product name: Recombinant human RBM47 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 444-593 aa of BC126261

  Sequence: VAIPAIGAQYSMFPAAPAPKMIEDGKIHTVEHMISPIAVQPDPASAAAAAAAAAAAAAAVIPTVSTPPPFQGRPITPVYTVAPNVQRIPTAGIYGASYVPFAAPATATIATLQKNAAAAAAVYGGYAGYIPQAFPAAAIQVPIPDVYQTY

• Full Name: RNA binding motif protein 47
• Calculated Molecular Weight: 593 aa, 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC126261
• Gene Symbol: RBM47
• Gene ID (NCBI): 54502
• RRID: AB_2879356
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: A0AV96
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Death Discov; RBM47 inhibits hepatocellular carcinoma progression by targeting UPF1 as a DNA/RNA regulator.; Authors - Tao Guo ; KD Validated
human	WB,IP	Apoptosis; Deubiquitinating enzyme OTUD4 stabilizes RBM47 to induce ATF3 transcription: a novel mechanism underlying the restrained malignant properties of ccRCC cells; Authors - Ziyao Li
mouse	WB	Korean J Physiol Pharmacol; Curcumin modulates the apolipoprotein B mRNA editing by coordinating the expression of cytidine deamination to uridine editosome components in primary mouse hepatocytes.; Authors - Pan He