RBM47 Recombinant monoclonal antibody

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• RBM47 Recombinant monoclonal antibody (86009-1-RR)

RBM47 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86009-1-RR Copied!

Clone No. 250436H8

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

NET18, RNA binding motif protein 47, RNA binding protein 47, RNA-binding motif protein 47, RNA-binding protein 47

• 

  WB analysis using 86009-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86009-1-RR (RBM47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A549 using 86009-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using RBM47 antibody (86009-1-RR, Clone: 250436H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 86009-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86009-1-RR against Human RBM47 were performed. The affinity constant is 1.02 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: MCF-7 cells, A549 cells, Caco-2 cells
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86009-1-RR targets RBM47 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag20984

  Product name: Recombinant human RBM47 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 444-593 aa of BC126261

  Sequence: VAIPAIGAQYSMFPAAPAPKMIEDGKIHTVEHMISPIAVQPDPASAAAAAAAAAAAAAAVIPTVSTPPPFQGRPITPVYTVAPNVQRIPTAGIYGASYVPFAAPATATIATLQKNAAAAAAVYGGYAGYIPQAFPAAAIQVPIPDVYQTY

• Full Name: RNA binding motif protein 47
• Calculated Molecular Weight: 593 aa, 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC126261
• Gene Symbol: RBM47
• Gene ID (NCBI): 54502
• RRID: AB_3744545
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: A0AV96
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM47, also known as RNA-binding motif protein 47 or RNA-binding protein 47, is a vertebrate-conserved RBP containing three RNA-recognition motifs (RRMs) that perform multifaceted roles in RNA editing, early embryonic development, and cancer.Recent studies suggest that RBM47 functions as a tumor suppressor. It has been shown to suppress the progression of breast and colon cancers. RBM47 represents a molecular switch of cell fate decisions that functions as a regulator of the p53/p21-signaling axis. (PMID: 34499322, PMID: 31511650)

Publications

What published studies show

The RBM47 Recombinant monoclonal antibody (250436H8), catalog number 86009-1-RR, has 1 total citation. The cited publication appeared in International Journal of Biological Macromolecules (Impact Factor 8.7) and investigated the oncogenic function of CCDC12 in regulating RBM47 splicing in breast cancer. The research field is breast cancer biology and RNA splicing regulation. The antibody was applied via IHC in human samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Int J Biol Macromol; Unveiling the oncogenic function of CCDC12 in regulating RBM47 splicing in breast cancer.; Authors - Zhoujie Ye ; KD Validated