RBM47 Recombinant monoclonal antibody

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  WB analysis using 86009-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86009-2-RR (RBM47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86009-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86009-2-RR against Human RBM47 were performed. The affinity constant is 1.40 nM.

RBM47 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86009-2-RR

Clone No.250436D5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

NET18, RNA binding motif protein 47, RNA binding protein 47, RNA-binding motif protein 47, RNA-binding protein 47

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Tested Applications

• Positive WB detected in: MCF-7 cells, A549 cells, Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86009-2-RR targets RBM47 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag20984

  Product name: Recombinant human RBM47 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 444-593 aa of BC126261

  Sequence: VAIPAIGAQYSMFPAAPAPKMIEDGKIHTVEHMISPIAVQPDPASAAAAAAAAAAAAAAVIPTVSTPPPFQGRPITPVYTVAPNVQRIPTAGIYGASYVPFAAPATATIATLQKNAAAAAAVYGGYAGYIPQAFPAAAIQVPIPDVYQTY

• Full Name: RNA binding motif protein 47
• Calculated Molecular Weight: 593 aa, 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC126261
• Gene Symbol: RBM47
• Gene ID (NCBI): 54502
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: A0AV96
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM47, also known as RNA-binding motif protein 47 or RNA-binding protein 47, is a vertebrate-conserved RBP containing three RNA-recognition motifs (RRMs) that perform multifaceted roles in RNA editing, early embryonic development, and cancer.Recent studies suggest that RBM47 functions as a tumor suppressor. It has been shown to suppress the progression of breast and colon cancers. RBM47 represents a molecular switch of cell fate decisions that functions as a regulator of the p53/p21-signaling axis. (PMID: 34499322, PMID: 31511650)