RBM48 Polyclonal antibody

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• RBM48 Polyclonal antibody (28307-1-AP)

• 

  WB analysis using 28307-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28307-1-AP (RBM48 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 28307-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using RBM48 antibody (28307-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

RBM48 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 28307-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

C7orf64, UPF0712 protein C7orf64, RNA-binding protein 48, HSPC304

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse testis tissue
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28307-1-AP targets RBM48 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27277

  Product name: Recombinant human C7orf64 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 68-367 aa of BC104865

  Sequence: LYGAIEQYNALDEYPAEDFTEVYLIKFMNLQSARTAKRKMDEQSFFGGLLHVCYAPEFETVEETRKKLQMRKAYVVKTTENKDHYVTKKKLVTEHKDTEDFRQDFHSEMSGFCKAALNTSAGNSNPYLPYSCELPLCYFSSKCMCSSGGPVDRAPDSSKDGRNHHKTMGHYNHNDSLRKTQINSLKNSVACPGAQKAITSSEAVDRFMPRTTQLQERKRRREDDRKLGTFLQTNPTGNEIMIGPLLPDISKVDMHDDSLNTTANLIRHKLKEVISSVPKPPEDKPEDVHTSHPLKQRRRI

• Full Name: chromosome 7 open reading frame 64
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC104865
• Gene Symbol: C7orf64
• Gene ID (NCBI): 84060
• RRID: AB_3742291
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5RL73
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM48 (RNA Binding Motif Protein 48) is a gene encoding an RNA-binding protein, belonging to the RNA-binding motif protein family, which plays an important role in the splicing process of U12-type introns. Deletion of RBM48 leads to an increase in the retention of U12-type introns, which affects cell differentiation and proliferation. And mutations in RBM48 are associated with a variety of diseases. For example, U12 intron splicing defects are associated with myelodysplastic syndromes (MDS) and may increase the risk of acute myeloid leukemia (AML). RBM48 may serve as a prognostic marker in certain cancers, such as renal smoky cell carcinoma, renal clear cell carcinoma, and hepatocellular carcinoma.