RBM4B Polyclonal antibody

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• RBM4B Polyclonal antibody (15412-1-AP)

RBM4B Polyclonal Antibody for WB, IP, ELISA

Cat No. 15412-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

RBM30, RBM4B, RBM4L, RNA binding motif protein 30, RNA binding motif protein 4B, RNA binding protein 30, RNA binding protein 4B, ZCCHC15, ZCRB3B

• 

  WB analysis of HEK-293 using 15412-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15412-1-AP (RBM4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HEK-293 using 15412-1-AP

  IP result of anti-RBM4B (IP:15412-1-AP, 3ug; Detection:15412-1-AP 1:300) with HEK-293 cells lysate 1000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IP detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15412-1-AP targets RBM4B in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7679

  Product name: Recombinant human RBM4B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-359 aa of BC003503

  Sequence: MVKLFIGNLPREATEQEIRSLFEQYGKVLECDIIKNYGFVHIEDKTAAEDAIRNLHHYKLHGVNINVEASKNKSKASTKLHVGNISPTCTNQELRAKFEEYGPVIECDIVKDYAFVHMERAEDAVEAIRGLDNTEFQGKRMHVQLSTSRLRTAPGMGDQSGCYRCGKEGHWSKECPVDRTGRVADFTEQYNEQYGAVRTPYTMGYGESMYYNDAYGALDYYKRYRVRSYEAVAAAAAASAYNYAEQTMSHLPQVQSTTVTSHLNSTSVDPYDRHLLPNSGAAATSAAMAAAAATTSSYYGRDRSPLRRAAAMLPTVGEGYGYGPESELSQASAATRNSLYDMARYEREQYVDRARYSAF

• Full Name: RNA binding motif protein 4B
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 36-40 kDa
• GenBank Accession Number: BC003503
• Gene Symbol: RBM4B
• Gene ID (NCBI): 83759
• RRID: AB_2181210
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BQ04
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The RBM4B Polyclonal antibody (Cat# 15412-1-AP) has 2 citations. One appears in Cell Death Dis (IF 12.2), studying OTUD6B-mediated deubiquitination of FXR1 and MEK2/ERK signaling in colorectal cancer liver metastasis. The other is in Oncogene (IF 9.1), investigating RBM30 recruitment of DOT1L to activate STAT1 transcription and drive immune evasion in hepatocellular carcinoma. Both citations use the antibody for Western blot in human samples, reflecting its application in cancer biology and signaling pathway research.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Death Dis; OTUD6B-mediated K48 deubiquitination of FXR1 forms a positive feedback loop activating MEK2/ERK signaling in colorectal cancer liver metastasis.; Authors - Ying Lu
	WB	Oncogene; RBM30 recruits DOT1L to activate STAT1 transcription and drive immune evasion in hepatocellular carcinoma; Authors - Hexu Han