Tested Applications
| Positive WB detected in | HEK-293T cells, HepG2 cells, HEK-293 cells, Jurkat cells, NCCIT cells, HeLa cells, Neuro-2a cells |
| Positive IP detected in | HepG2 cells, K-562 cells |
| Positive IHC detected in | human breast cancer tissue, human placenta tissue, mouse brain tissue, human ovary tumor tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12498-1-AP targets FOX2/RBM9 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3168 Product name: Recombinant human RBM9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 6-358 aa of BC013115 Sequence: MVTQGNQEPTTTPDAMVQPFTTIPFPPPPQNGIPTEYGVPHTQDYAGQTGEHNLTLYGSTQAHGEQSSNSPSTQNGSLTTEGGAQTDGQQSQTQSSENSESKSTPKRLHVSNIPFRFRDPDLRQMFGGFGFVTFENSADADRAREKLHGTVVEGRKIEVNNATARVMTNKKMVTPYANGWKLSPVVGAVYGPELYAASSFQADVSLGNDAAVPLSGRGGINTYIPLIIPGFPYPTAATTAAAFRGAHLRGRGRTVYGAVRAVPPTAIPAYPGVVYQDGFYGADLYGGYAAYRYAQPATATAATAAAAAVAAYSDGYGRVYTADPYHALAPAASYGVGAVASLYRGGYSRFAPY Predict reactive species |
| Full Name | RNA binding motif protein 9 |
| Calculated Molecular Weight | 390 aa, 41 kDa |
| Observed Molecular Weight | 50- 60 kDa |
| GenBank Accession Number | BC013115 |
| Gene Symbol | RBM9 |
| Gene ID (NCBI) | 23543 |
| RRID | AB_2877861 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43251 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RBM9, also named as RBFOX2 and FOX2, is a RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. There are several isoforms of RBM9 produced by variable use of multiple alternatively spliced internal exons and alternative splice sites. RBM9 may play an important role in tissue specific alternative splicing regulation.(PMID: 19688295, 21623373, 16537540)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FOX2/RBM9 antibody 12498-1-AP | Download protocol |
| IHC protocol for FOX2/RBM9 antibody 12498-1-AP | Download protocol |
| IP protocol for FOX2/RBM9 antibody 12498-1-AP | Download protocol |
| WB protocol for FOX2/RBM9 antibody 12498-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
RBFOX2 antibody (Cat# 12498-1-AP) has 6 citations across Nature Cell Biology, Molecular Psychiatry, Cell Reports, JCI Insight, Frontiers in Pharmacology, and iScience. Research fields include RNA modification (m6A, 5hmC), cancer biology (leukemia, glioma, hepatocellular carcinoma, pan-cancer), neurodevelopment (ataxia, autism), vascular biology, and immunology (NETosis). Applications span WB, IF, IHC, and CoIP in human, mouse, and rat samples.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol RBFOX2 recognizes N6-methyladenosine to suppress transcription and block myeloid leukaemia differentiation | ||
Mol Psychiatry Bcl11a deficiency in cerebellar Purkinje cells causes ataxia and autistic-like behavior by altering Vav3. | ||
JCI Insight Vascular smooth muscle RbFox2 regulates the cytoskeleton and arterial stiffness by a RhoBTB1-Cullin-3 mechanism.
| ||
Front Pharmacol Integrated pan-cancer analysis reveals the immunological and prognostic potential of RBFOX2 in human tumors | ||
iScience Hypoxia induces hepatocellular carcinoma metastasis via the HIF-1α/METTL16/lnc-CSMD1-7/RBFOX2 axis |
Reviews
What customers say
All three reviews are 5-star ratings. Users tested the antibody on rat A7R5 smooth muscle cells at dilutions of 1:3000–1:4000 for Western Blot and Immunoprecipitation. Reviewers consistently highlight excellent specificity with no non-specific bands, reliable detection of endogenous protein, and results that meet or exceed expectations in both WB and co-IP applications. Overall, the antibody is described as performing perfectly as described, with high-quality and reproducible results.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Gaurav (Verified Customer) (12-19-2023) | Excellent antibody without non-specific bands. Works perfectly as described.
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Gaurav (Verified Customer) (10-04-2023) | Excellent antibody for endogenous proteins and works very fine. The quality of results is up to the expectations in co-immunoprecipitation and western blotting.
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Gaurav (Verified Customer) (10-04-2023) | Excellent antibody for endogenous proteins and works very fine. The quality of results is up to the expectations in co-immunoprecipitation and western blotting.
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