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  • KD/KO Validated

RBP1 Polyclonal antibody

RBP1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22683-1-AP

Synonyms

CRABP I, CRBP, CRBP I, CRBP1, CRBPI, RBP1, RBPC, Retinol binding protein 1



Tested Applications

Positive WB detected inmouse liver tissue, HEK-293 cells, human brain tissue, human liver tissue
Positive IHC detected inhuman pancreas tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22683-1-AP is RBP1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBP1 fusion protein Ag18572
Full Name retinol binding protein 1, cellular
Calculated molecular weight 135 aa, 16 kDa
Observed molecular weight 14-21 kDa
GenBank accession numberBC121052
Gene symbol RBP1
Gene ID (NCBI) 5947
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RBP1 antibody 22683-1-APDownload protocol
IHC protocol for RBP1 antibody 22683-1-APDownload protocol
IF protocol for RBP1 antibody 22683-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

Cell Death Dis

The RBP1-CKAP4 axis activates oncogenic autophagy and promotes cancer progression in oral squamous cell carcinoma.

Authors - Ling Gao
  • KD Validated
mouseWB

J Food Biochem

Inhibitory effect of chitooligosaccharides on retinol metabolism and bioavailability in mice.

Authors - Wei Li
mouseIHC

Metabolism

Disruption of retinoid homeostasis induces RBP4 overproduction in diabetes: O-GlcNAcylation involved.

Authors - Shyi-Jang Shin