CoraLite®594-conjugated RBP4 Monoclonal antibody
RBP4 Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
IF
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1D12B11
Cat no : CL594-66104
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | human liver cancer tissue |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of CL594-66104 is RBP4 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | RBP4 fusion protein Ag19295 |
Full Name | retinol binding protein 4, plasma |
Calculated molecular weight | 201 aa, 23 kDa |
Observed molecular weight | 23 kDa |
GenBank accession number | BC020633 |
Gene symbol | RBP4 |
Gene ID (NCBI) | 5950 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RBP4 (retinol-binding protein 4) is a carrier protein that transports vitamin A (retinol) from the liver to the peripheral tissues. Synthesized primarily by hepatocytes and adipocytes as a 21 kDa non-glycosylated protein, RBP4 is secreted into the circulation as a retinol-RBP4 complex. In plasma the RBP4-retinol complex is bound to transthyretin (TRR), which prevents prevent kidney filtration. Two truncated forms of RBP4, RBP4-L (truncated at Leu-183) and RBP4-LL (truncated at Leu-182 and Leu-183), exist by proteolytic process. RBP4-L and RBP4-LL, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. Urinary RBP4 has been reported as marker for glomerular disease. RBP4 also was identified as an adipokine that elevated in some INS-resistant states. Measurement of serum RBP4 could be used to assess the risk of INS resistance, type 2 diabetes, obesity, and cardiovascular disease. (18752671, 16034410)
Protocols
Product Specific Protocols | |
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IF protocol for CL594 RBP4 antibody CL594-66104 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |