RBP4 Monoclonal antibody

RBP4 Monoclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, FC,ELISA

Conjugate

Unconjugated

CloneNo.

1D12B11

Cat no : 66104-1-Ig

Synonyms

Plasma retinol binding protein, PRBP, RBP, RBP4, Retinol binding protein 4



Tested Applications

Positive WB detected inHuman Blood, tissue
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66104-1-Ig is RBP4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RBP4 fusion protein Ag19295
Full Name retinol binding protein 4, plasma
Calculated molecular weight 201 aa, 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC020633
Gene symbol RBP4
Gene ID (NCBI) 5950
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBP4 (retinol-binding protein 4) is a carrier protein that transports vitamin A (retinol) from the liver to the peripheral tissues. Synthesized primarily by hepatocytes and adipocytes as a 21 kDa non-glycosylated protein, RBP4 is secreted into the circulation as a retinol-RBP4 complex. In plasma the RBP4-retinol complex is bound to transthyretin (TRR), which prevents prevent kidney filtration. Two truncated forms of RBP4, RBP4-L (truncated at Leu-183) and RBP4-LL (truncated at Leu-182 and Leu-183), exist by proteolytic process. RBP4-L and RBP4-LL, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. Urinary RBP4 has been reported as marker for glomerular disease. RBP4 also was identified as an adipokine that elevated in some INS-resistant states. Measurement of serum RBP4 could be used to assess the risk of INS resistance, type 2 diabetes, obesity, and cardiovascular disease. (18752671, 16034410)

Protocols

Product Specific Protocols
WB protocol for RBP4 antibody 66104-1-IgDownload protocol
IHC protocol for RBP4 antibody 66104-1-IgDownload protocol
FC protocol for RBP4 antibody 66104-1-IgDownload protocol
Standard Protocols
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