RBPJ Polyclonal antibody

RBPJ Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30044-1-AP

Synonyms

CBF 1, CBF1, csl, IGKJRB, IGKJRB1, KBF2, RBP J, RBP J kappa, RBP JK, RBPJ, RBPJK, RBPSUH, SUH

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Validation Data Gallery

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  WB analysis of MCF-7 using 30044-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 30044-1-AP (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 30044-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 30044-1-AP (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 30044-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 30044-1-AP (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 30044-1-AP

  IP result of anti-RBPJ (IP:30044-1-AP, 4ug; Detection:30044-1-AP 1:1000) with HepG2 cells lysate 1080 ug.

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  IHC staining of human pancreas cancer using 30044-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 30044-1-AP (RBPJ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 30044-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 30044-1-AP (RBPJ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, MCF-7 cells, NIH/3T3 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30044-1-AP targets RBPJ in WB, IP, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBPJ fusion protein Ag32552
• Full Name: recombination signal binding protein for immunoglobulin kappa J region
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: ~60 kDa
• GenBank Accession Number: BC064976
• Gene Symbol: RBPJ
• Gene ID (NCBI): 3516
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBPJ, also named as IGKJRB, IGKJRB1, RBPJK, RBPSUH, NY-REN-30, CBF-1 and CSL, belongs to the Su(H) family. It is a transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. RBPJ acts as a transcriptional repressor when it is not associated with Notch proteins. It is specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. RBPJ binds to the Runx3 promoter in the atrioventricular canal in vivo, and inhibition of Notch reduces RUNX3 expression in the cardiac cushion of embryonic hearts.