RBPJ Monoclonal antibody

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  WB analysis of HeLa using 66132-1-Ig

  WB result of RBPJ antibody (66132-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBPJ transfected HeLa cells.

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  WB analysis of HepG2 using 66132-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66132-1-Ig (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 66132-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66132-1-Ig (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

RBPJ Monoclonal Antibody for WB, ELISA

Cat No. 66132-1-Ig

Clone No.2F12G12

Publications(2)

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IP, ELISA

Synonyms

CBF 1, CBF1, csl, IGKJRB, IGKJRB1, KBF2, NY-REN-30, RBP J, RBP J kappa, RBP JK, RBPJ, RBPJK, RBPSUH, SUH

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Tested Applications

• Positive WB detected in: HepG2 cells, human brain tissue, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IP: See 1 publications below

Product Information

66132-1-Ig targets RBPJ in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6302

  Product name: Recombinant human RBPJ protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 138-486 aa of BC064976

  Sequence: DIGVFLSKRIKVISKPSKKKQSLKNADLCIASGTKVALFNRLRSQTVSTRYLHVEGGNFHASSQQWGAFFIHLLDDDESEGEEFTVRDGYIHYGQTVKLVCSVTGMALPRLIIRKVDKQTALLDADDPVSQLHKCAFYLKDTERMYLCLSQERIIQFQATPCPKEPNKEMINDGASWTIISTDKAEYTFYEGMGPVLAPVTPVPVVESLQLNGGGDVAMLELTGQNFTPNLRVWFGDVEAETMYRCGESMLCVVPDISAFREGWRWVRQPVQVPVTLVRNDGIIYSTSLTFTYTPEPGPRPHCSAAGAILRANSSQVPPNESNTNSEGSYTNASTNSTSVTSSTATVVS

• Full Name: recombination signal binding protein for immunoglobulin kappa J region
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC064976
• Gene Symbol: RBPJ
• Gene ID (NCBI): 3516
• RRID: AB_2881531
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q06330
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Cell Mol Med; Identification and validation of basement membrane-associated gene AGRN as prognostic and immune-associated biomarkers in colorectal cancer patients; Authors - Jianrong Li
mouse	WB,IP	Mol Med; Celastrol promotes DNA damage and apoptosis in uterine corpus endometrial carcinoma via promotion of KAT2B-mediated RBPJ acetylation and repression of MCM4 transcription; Authors - Lipeng Pei