RBPMS Recombinant antibody, PBS Only

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  WB analysis using 85086-5-RR (same clone as 85086-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85086-5-RR (RBPMS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85086-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 85086-5-RR (same clone as 85086-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85086-5-RR (RBPMS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85086-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 85086-5-RR (same clone as 85086-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85086-5-RR (RBPMS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85086-5-PBS in a different storage buffer formulation.

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  IF Staining of mouse retina using 85086-5-RR (same clone as 85086-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse retina tissue using RBPMS antibody (85086-5-RR, Clone: 242730B7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85086-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 85086-5-RR (same clone as 85086-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RBPMS antibody (85086-5-RR, Clone: 242730B7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85086-5-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 85086-5-RR (same clone as 85086-5-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug RBPMS Recombinant antibody (85086-5-RR, Clone:242730B7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85086-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85086-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85086-5-RR against Human RBPMS were performed. The affinity constant is 0.153 nM.

RBPMS Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Cat No. 85086-5-PBS

Clone No.242730B7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Synonyms

242730B7, Hermes, RBP MS, RBP-MS, RNA-binding protein with multiple splicing

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Product Information

85086-5-PBS targets RBPMS in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RBPMS fusion protein Ag7256
• Full Name: RNA binding protein with multiple splicing
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 28-30 kDa
• GenBank Accession Number: BC003608
• Gene Symbol: RBPMS
• Gene ID (NCBI): 11030
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q93062
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RBPMS is a member of the RNA recognition motif family of RNA-binding proteins.It has a single, putative RNA recognition motif in its N-terminus.