Multi-rAb® RBPMS Multi-Recombinant antibody

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• Multi-rAb® RBPMS Multi-Recombinant antibody (RMX00116)

RBPMS Multi-Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. RMX00116

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Hermes, RBP MS, RBP-MS, RNA-binding protein with multiple splicing

• 

  WB analysis using RMX00116

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00116 (RBPMS antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse eye using RMX00116

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using RMX00116 (RBPMS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse eye using RMX00116

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using RMX00116 (RBPMS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse eye using RMX00116

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using RBPMS antibody (RMX00116) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: MDA-MB-231 cells, mouse colon tissue, rat colon tissue
• Positive IHC detected in: mouse eye tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse eye tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00116 targets RBPMS in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: RNA binding protein with multiple splicing
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 28-30 kDa
• GenBank Accession Number: BC003608
• Gene Symbol: RBPMS
• Gene ID (NCBI): 11030
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: Q93062
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBPMS is a member of the RNA recognition motif family of RNA-binding proteins.It has a single, putative RNA recognition motif in its N-terminus.