|Positive WB detected in||HeLa cells, Jurkat cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14895-1-AP targets RBX1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat, Eriocheir sinensis|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RBX1 fusion protein Ag6685|
|Full Name||ring-box 1|
|Calculated molecular weight||12 kDa|
|Observed molecular weight||15 kDa|
|GenBank accession number||BC001466|
|Gene ID (NCBI)||9978|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
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Cullin1 binds and promotes NLRP3 ubiquitination to repress systematic inflammasome activation.
Expression pattern and functional analysis of the two RING box protein RBX in spermatogenesis of Chinese mitten crab Eriocheir sinensis.
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