RC3H1 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 84323-1-RR (same clone as 84323-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84323-1-RR (RC3H1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84323-1-PBS in a different storage buffer formulation.

RC3H1 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 84323-1-PBS

Clone No.241046G10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

241046G10, KIAA2025, RING finger protein 198, RNF198, ROQUIN

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Product Information

84323-1-PBS targets RC3H1 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: ring finger and CCCH-type zinc finger domains 1
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: NM_172071
• Gene Symbol: RC3H1
• Gene ID (NCBI): 149041
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q5TC82
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Roquin-1 (Rc3h1) is an E3 ubiquitin ligase originally discovered in a mutational screen for genetic factors contributory to systemic lupus erythematosus-like symptoms in mice. A single base pair mutation in the Rc3h1 gene resulted in the manifestation of autoantibody production and sustained immunological inflammation characterized by excessive T follicular helper cell activation and formation of germinal centers. (PMID: 26673963)