RCC2 Polyclonal antibody, PBS Only

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• RCC2 Polyclonal antibody, PBS Only (16755-1-PBS)

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  WB analysis of HeLa using 16755-1-AP (same clone as 16755-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16755-1-AP (RCC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.

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  WB analysis using 16755-1-AP (same clone as 16755-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16755-1-AP (RCC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 16755-1-AP (same clone as 16755-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16755-1-AP (RCC2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293T using 16755-1-AP (same clone as 16755-1-PBS)

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 16755-1-AP (RCC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 16755-1-AP (same clone as 16755-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 16755-1-AP (same clone as 16755-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 16755-1-AP (same clone as 16755-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 16755-1-AP (same clone as 16755-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 16755-1-AP (same clone as 16755-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 16755-1-AP (same clone as 16755-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 16755-1-AP (same clone as 16755-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 16755-1-AP (same clone as 16755-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.

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  IHC staining of rat colon using 16755-1-AP (same clone as 16755-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 16755-1-AP (same clone as 16755-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 16755-1-AP (same clone as 16755-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 16755-1-AP (same clone as 16755-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.

RCC2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 16755-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

16755-1-PBS targets RCC2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10271

  Product name: Recombinant human RCC2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 168-522 aa of BC042141

  Sequence: EGKLWSWGRNEKGQLGHGDTKRVEAPRLIEGLSHEVIVSAACGRNHTLALTETGSVFAFGENKMGQLGLGNQTDAVPSPAQIMYNGQPITKMACGAEFSMIMDCKGNLYSFGCPEYGQLGHNSDGKFIARAQRIEYDCELVPRRVAIFIEKTKDGQILPVPNVVVRDVACGANHTLVLDSQKRVFSWGFGGYGRLGHAEQKDEMVPRLVKLFDFPGRGASQIYAGYTCSFAVSEVGGLFFWGATNTSRESTMYPKAVQDLCGWRIRSLACGKSSIIVAADESTISWGPSPTFGELGYGDHKPKSSTAAQEVKTLDGIFSEQVAMGYSHSLVIARDESETEKEKIKKLPEYNPRTL

• Full Name: regulator of chromosome condensation 2
• Calculated Molecular Weight: 522 aa, 56 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC042141
• Gene Symbol: RCC2
• Gene ID (NCBI): 55920
• RRID: AB_2878311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9P258
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RCC2 (also known as TD60) is a guanine exchange factor that is active on RalA, a small GTPase. RCC2 and RalA are both essential for proper kinetochore-microtubule function in early mitosis. RCC2 has been shown to be a biomarker for colorectal cancer.