Pirh2 Polyclonal antibody

• Featured Product
• KD/KO Validated

Pirh2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13820-1-AP

Synonyms

ARNIP, CHIMP, hARNIP, hPirh2, p53 induced RING H2 protein, Pirh2, RCHY1, RING finger protein 199, RNF199, Zinc finger protein 363, ZNF363

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Validation Data Gallery

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  WB analysis using 13820-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13820-1-AP (Pirh2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13820-1-AP

  WB result of Pirh2 antibody (13820-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Pirh2 transfected HEK-293 cells.

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  IHC staining of mouse liver using 13820-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13820-1-AP (Pirh2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 13820-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13820-1-AP (Pirh2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse spleen tissue, HEK-293 cells, mouse testis tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

13820-1-AP targets Pirh2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Pirh2 fusion protein Ag4916
• Full Name: ring finger and CHY zinc finger domain containing 1
• Calculated Molecular Weight: 261 aa, 30 kDa
• Observed Molecular Weight: 45 KDa, 30 kDa
• GenBank Accession Number: BC047393
• Gene Symbol: RCHY1
• Gene ID (NCBI): 25898
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pirh2 is also named as RCHY1, ARNIP, CHIMP, PIRH2, RNF199, ZNF363 and has p53-induced ubiquitin-protein ligase activity, promoting p53 degradation. Pirh2 monoubiquitinates the translesion DNA polymerase POLH and contributes to the regulation of the cell cycle progression. This protein has 8 isoforms produced by alternative splicing and can exsit as a monomer or homodimer.

Publications

Species	Application	Title
human	WB	Sci Adv; Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.; Authors - Pengbo Cao ; KD Validated
human	WB,IHC	Sci Adv; G6PD-mediated increase in de novo NADP+ biosynthesis promotes antioxidant defense and tumor metastasis.; Authors - Yang Zhang
human	WB	J Agric Food Chem; Avenanthramide A induces cellular senescence via miR-129-3p/Pirh2/p53 signaling pathway to suppress colon cancer growth.; Authors - Rong Fu ; KD Validated

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