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Pirh2 Polyclonal antibody

Pirh2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13820-1-AP

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

RCHY1, Androgen receptor N-terminal-interacting protein, ARNIP, CHIMP, CH-rich-interacting match with PLAG1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis using 13820-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13820-1-AP (Pirh2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 13820-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13820-1-AP (Pirh2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse liver using 13820-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13820-1-AP (Pirh2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse liver using 13820-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13820-1-AP (Pirh2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse stomach using 13820-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13820-1-AP (Pirh2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse adrenal gland using 13820-1-AP
Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 13820-1-AP (Pirh2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat adrenal gland using 13820-1-AP
Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 13820-1-AP (Pirh2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat kidney using 13820-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13820-1-AP (Pirh2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse spleen tissue, HL-60 cells, rat testis tissue, mouse testis tissue
Positive IHC detected in	mouse liver tissue, rat adrenal gland tissue, mouse stomach tissue, mouse adrenal gland tissue, rat kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13820-1-AP targets Pirh2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4916 Product name: Recombinant human RCHY1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-261 aa of BC047393 Sequence: MAATAREDGASGQERGQRGCEHYDRGCLLKAPCCDKLYTCRLCHDNNEDHQLDRFKVKEVQCINCEKIQHAQQTCEECSTLFGEYYCDICHLFDKDKKQYHCENCGICRIGPKEDFFHCLKCNLCLAMNLQGRHKCIENVSQQNCPICLEDIHTSRVVAHVLPCGHLLHRTCYEEMLKEGYRCPLCMHSALDMTRYWRQLDDEVAQTPMPSEYQNMTVDILCNDCNGRSTVQFHILGMKCKICESYNTAQAGGRRISLDQQ Predict reactive species
Full Name	ring finger and CHY zinc finger domain containing 1
Calculated Molecular Weight	261 aa, 30 kDa
Observed Molecular Weight	45 KDa, 30 kDa
GenBank Accession Number	BC047393
Gene Symbol	RCHY1
Gene ID (NCBI)	25898
RRID	AB_2891219
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96PM5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pirh2 is also named as RCHY1, ARNIP, CHIMP, PIRH2, RNF199, ZNF363 and has p53-induced ubiquitin-protein ligase activity, promoting p53 degradation. Pirh2 monoubiquitinates the translesion DNA polymerase POLH and contributes to the regulation of the cell cycle progression. This protein has 8 isoforms produced by alternative splicing and can exsit as a monomer or homodimer.

Protocols

Product Specific Protocols
IHC protocol for Pirh2 antibody 13820-1-AP	Download protocol
WB protocol for Pirh2 antibody 13820-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The Pirh2 Polyclonal antibody (Cat# 13820-1-AP) has been cited in 3 publications. Citations appear in Science Advances (2 papers) and Journal of Agricultural and Food Chemistry (1 paper). The associated research fields include hepatocellular carcinoma and p53 signaling, tumor metastasis and antioxidant defense via G6PD-mediated NADP+ biosynthesis, and colon cancer suppression through cellular senescence involving the miR-129-3p/Pirh2/p53 pathway.

Species	Application	Title
human	WB,IHC	Sci Adv G6PD-mediated increase in de novo NADP+ biosynthesis promotes antioxidant defense and tumor metastasis. Authors - Yang Zhang View Article
human	WB	Sci Adv Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling. Authors - Pengbo Cao KD Validated View Article
human	WB	J Agric Food Chem Avenanthramide A induces cellular senescence via miR-129-3p/Pirh2/p53 signaling pathway to suppress colon cancer growth. Authors - Rong Fu KD Validated View Article