RCOR3 Polyclonal antibody

RCOR3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 55173-1-AP

Synonyms

RCOR 3

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Validation Data Gallery

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  WB analysis of A431 using 55173-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55173-1-AP (RCOR3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 55173-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using RCOR3 antibody (55173-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: A431 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55173-1-AP targets RCOR3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: REST corepressor 3
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 63-70 kDa
• GenBank Accession Number: NM_018254
• Gene Symbol: RCOR3
• Gene ID (NCBI): 55758
• RRID: AB_2881281
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.