Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, mouse brain tissue, mouse eye tissue, mouse placenta tissue, mouse kidney tissue, rat kidney tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse testis tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10705-1-AP targets NELFE in WB, IHC, IF/ICC, FC (Intra), IP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1186 Product name: Recombinant human RDBP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-333 aa of BC011600 Sequence: MLVIPPGLSEEEEALQKKFNKLKKKKKALLALKKQSSSSTTSQGGVKRSLSEQPVMDTATATEQAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSISADDDLQESSRRPQRKSLYESDRLRELGPDGEEAEGPGAGDGPPRSFDWGYEERSGAHSSASPPRSRSRDRSHERNRDRDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDVYKENLVDGF Predict reactive species |
| Full Name | RD RNA binding protein |
| Calculated Molecular Weight | 37 kDa, 43 kDa |
| Observed Molecular Weight | 43 kDa |
| GenBank Accession Number | BC011600 |
| Gene Symbol | NELFE |
| Gene ID (NCBI) | 7936 |
| RRID | AB_513966 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P18615 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RDBP, also named NELF-E and RD, belongs to the RRM NELF-E family. It is an essential component of the NELF complex which is a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, a complex acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. RDBP was recently found implicated in RNA polymerase II pausing by association with Hsp90. Catalog# 10705-1-AP is a widely used rabbit polyclonal antibody obtained by immunizing animals with full-length RDBP of human origin.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for NELFE antibody 10705-1-AP | Download protocol |
| IF protocol for NELFE antibody 10705-1-AP | Download protocol |
| IHC protocol for NELFE antibody 10705-1-AP | Download protocol |
| IP protocol for NELFE antibody 10705-1-AP | Download protocol |
| WB protocol for NELFE antibody 10705-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NELFE (RDBP) polyclonal antibody (Cat# 10705-1-AP) has 23 citations spanning high-impact journals including Nature, Cell, Nature Communications, PNAS, Nucleic Acids Research, and Development. Research fields covered include RNA Polymerase II transcriptional pausing, gene regulation, inflammation, cancer (gastric, oral, hepatocellular), HIV reactivation, neurobiology, muscle repair, endometrial function, adipogenesis, intestinal barrier integrity, and epigenetics. Applications cited include WB, IHC, IF, ChIP, and RIP across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
Cell Hsp90 globally targets paused RNA polymerase to regulate gene expression in response to environmental stimuli. | ||
Nat Commun Negative elongation factor complex enables macrophage inflammatory responses by controlling anti-inflammatory gene expression. | ||
Nat Commun RNA polymerase II pausing factor NELF in CD8+ T cells promotes antitumor immunity. | ||
Nucleic Acids Res Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.
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Reviews
What customers say
The single available review rates this antibody 5 stars. The user reported excellent performance in Western Blot at a 1:1000 dilution on mouse embryonic fibroblasts, noting that it produced a clean single band. Overall, the feedback highlights strong specificity and reliable results for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
HUAI-CHIN (Verified Customer) (02-14-2019) | This antibody works great in Western. Gives me clean single band.
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