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NELFE Polyclonal antibody
NELFE Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 10705-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, mouse brain tissue, mouse eye tissue, mouse placenta tissue, rat brain tissue, HeLa cells, mouse kidney tissue, rat kidney tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10705-1-AP targets NELFE in WB, RIP, IP, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NELFE fusion protein Ag1186|
|Full Name||RD RNA binding protein|
|Calculated molecular weight||37 kDa, 43 kDa|
|Observed molecular weight||43 kDa|
|GenBank accession number||BC011600|
|Gene ID (NCBI)||7936|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
RDBP, also named NELF-E and RD, belongs to the RRM NELF-E family. It is an essential component of the NELF complex which is a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, a complex acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. RDBP was recently found implicated in RNA polymerase II pausing by association with Hsp90. Catalog# 10705-1-AP is a widely used rabbit polyclonal antibody obtained by immunizing animals with full-length RDBP of human origin.
|Product Specific Protocols|
|WB protocol for NELFE antibody 10705-1-AP||Download protocol|
|IHC protocol for NELFE antibody 10705-1-AP||Download protocol|
|IF protocol for NELFE antibody 10705-1-AP||Download protocol|
|IP protocol for NELFE antibody 10705-1-AP||Download protocol|
|Click here to view our Standard Protocols|
The landscape of RNA Pol II binding reveals a stepwise transition during ZGA.
Hsp90 globally targets paused RNA polymerase to regulate gene expression in response to environmental stimuli.
Negative elongation factor complex enables macrophage inflammatory responses by controlling anti-inflammatory gene expression.
RNA polymerase II pausing factor NELF in CD8+ T cells promotes antitumor immunity.
Negative elongation factor regulates muscle progenitor expansion for efficient myofiber repair and stem cell pool repopulation.
Proc Natl Acad Sci U S A
Immediate mediators of the inflammatory response are poised for gene activation through RNA polymerase II stalling.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
HUAI-CHIN (Verified Customer) (02-14-2019)
This antibody works great in Western. Gives me clean single band.