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  • KD/KO Validated

NELFE Antibody

NELFE Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, chIP, ELISA

Conjugate

Unconjugated

Cat no : 10705-1-AP

Synonyms

D6S45, Negative elongation factor E, NELF E, NELFE, RD, RD RNA binding protein, RDBP, RDP, RNA binding protein RD



Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue, HeLa cells, mouse eye tissue, rat brain tissue, mouse placenta tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10705-1-AP targets NELFE in WB, IP, IHC, IF, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NELFE fusion protein Ag1186
Full Name RD RNA binding protein
Calculated molecular weight 37 kDa,43 kDa
Observed molecular weight 43 kDa
GenBank accession number BC011600
Gene symbol RDBP
Gene ID (NCBI) 7936
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RDBP, also named as NELF-E and RD, belongs to the RRM NELF-E family. It is an essential component of the NELF complex which is a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, a complex acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. RDBP was recently found implicated in RNA polymerase II pausing by association with Hsp90. Catalog# 10705-1-AP is a widely used rabbit polyclonal antibdoy obtained by immunizing animals with full-length RDBP of human origin.

Protocols

Product Specific Protocols
WB protocol for NELFE antibody 10705-1-APDownload protocol
IHC protocol for NELFE antibody 10705-1-APDownload protocol
IF protocol for NELFE antibody 10705-1-APDownload protocol
IP protocol for NELFE antibody 10705-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Oncol Rep

Overexpression of the RD RNA binding protein in hepatitis C virus-related hepatocellular carcinoma.

Authors - Iida Michihisa M
  • KD Validated
ratWB, IF

Neurobiol Dis

Striatal NELF-mediated RNA polymerase II stalling controls l-dopa induced dyskinesia.

Authors - Matthieu F Bastide
mouseIHC,WB

FASEB J

Negative elongation factor is essential for endometrial function.

Authors - Sylvia C Hewitt
mouseWB,chIP

Elife

Gene-specific mechanisms direct glucocorticoid-receptor-driven repression of inflammatory response genes in macrophages.

Authors - Maria A Sacta
humanWB

Nucleic Acids Res

Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.

Authors - Xiaodong Lu
  • KD Validated
mouseWB,ChIP

Proc Natl Acad Sci U S A

Glucocorticoid receptor represses proinflammatory genes at distinct steps of the transcription cycle.

Authors - Gupte Rebecca R

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

HUAI-CHIN (Verified Customer) (02-14-2019)

This antibody works great in Western. Gives me clean single band.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse embryonic fibroblast
No image submitted with review