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NELFE Polyclonal antibody

NELFE Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 10705-1-AP


D6S45, Negative elongation factor E, NELF E, NELFE, RD, RD RNA binding protein, RDBP, RDP, RNA binding protein RD

Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue, mouse eye tissue, mouse placenta tissue, rat brain tissue, HeLa cells, mouse kidney tissue, rat kidney tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10705-1-AP targets NELFE in WB, IP, IF, FC, RIP, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NELFE fusion protein Ag1186
Full Name RD RNA binding protein
Calculated Molecular Weight 37 kDa, 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC011600
Gene Symbol RDBP
Gene ID (NCBI) 7936
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RDBP, also named NELF-E and RD, belongs to the RRM NELF-E family. It is an essential component of the NELF complex which is a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, a complex acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. RDBP was recently found implicated in RNA polymerase II pausing by association with Hsp90. Catalog# 10705-1-AP is a widely used rabbit polyclonal antibody obtained by immunizing animals with full-length RDBP of human origin.


Product Specific Protocols
WB protocol for NELFE antibody 10705-1-APDownload protocol
IHC protocol for NELFE antibody 10705-1-APDownload protocol
IF protocol for NELFE antibody 10705-1-APDownload protocol
IP protocol for NELFE antibody 10705-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




The landscape of RNA Pol II binding reveals a stepwise transition during ZGA.

Authors - Bofeng Liu
human, mouseWB


Hsp90 globally targets paused RNA polymerase to regulate gene expression in response to environmental stimuli.

Authors - Sawarkar Ritwick R

Nat Commun

Negative elongation factor complex enables macrophage inflammatory responses by controlling anti-inflammatory gene expression.

Authors - Li Yu

Nat Commun

RNA polymerase II pausing factor NELF in CD8+ T cells promotes antitumor immunity.

Authors - Bogang Wu

Dev Cell

Negative elongation factor regulates muscle progenitor expansion for efficient myofiber repair and stem cell pool repopulation.

Authors - Daniel C L Robinson

Proc Natl Acad Sci U S A

Immediate mediators of the inflammatory response are poised for gene activation through RNA polymerase II stalling.

Authors - Adelman Karen K


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


HUAI-CHIN (Verified Customer) (02-14-2019)

This antibody works great in Western. Gives me clean single band.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse embryonic fibroblast