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NELFE Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 10705-1-AP
D6S45, Negative elongation factor E, NELF E, NELFE, RD, RD RNA binding protein, RDBP, RDP, RNA binding protein RD
|Positive WB detected in||HEK-293 cells, mouse brain tissue, HeLa cells, mouse eye tissue, rat brain tissue, mouse placenta tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10705-1-AP targets NELFE in WB, IP, IHC, IF, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NELFE fusion protein Ag1186|
|Full Name||RD RNA binding protein|
|Calculated molecular weight||37 kDa,43 kDa|
|Observed molecular weight||43 kDa|
|GenBank accession number||BC011600|
|Gene ID (NCBI)||7936|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RDBP, also named as NELF-E and RD, belongs to the RRM NELF-E family. It is an essential component of the NELF complex which is a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, a complex acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. RDBP was recently found implicated in RNA polymerase II pausing by association with Hsp90. Catalog# 10705-1-AP is a widely used rabbit polyclonal antibdoy obtained by immunizing animals with full-length RDBP of human origin.
|Product Specific Protocols|
|WB protocol for NELFE antibody 10705-1-AP||Download protocol|
|IHC protocol for NELFE antibody 10705-1-AP||Download protocol|
|IF protocol for NELFE antibody 10705-1-AP||Download protocol|
|IP protocol for NELFE antibody 10705-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Overexpression of the RD RNA binding protein in hepatitis C virus-related hepatocellular carcinoma.
Striatal NELF-mediated RNA polymerase II stalling controls l-dopa induced dyskinesia.
Negative elongation factor is essential for endometrial function.
Gene-specific mechanisms direct glucocorticoid-receptor-driven repression of inflammatory response genes in macrophages.
Nucleic Acids Res
Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.
Proc Natl Acad Sci U S A
Glucocorticoid receptor represses proinflammatory genes at distinct steps of the transcription cycle.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
HUAI-CHIN (Verified Customer) (02-14-2019)
This antibody works great in Western. Gives me clean single band.