RDH11 Polyclonal antibody

RDH11 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11077-1-AP

Synonyms

ARSDR1, CGI 82, HCBP12, MDT1, PSDR1, RALR1, RDH11, Retinal reductase 1, Retinol dehydrogenase 11, SCALD, SDR7C1

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Validation Data Gallery

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  IHC staining of human prostate cancer using 11077-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11077-1-AP (RDH11 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 11077-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11077-1-AP (RDH11 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 11077-1-AP

  Immunofluorescent analysis of PC-3 cells using 11077-1-AP (RDH11 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: PC-3 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11077-1-AP targets RDH11 in IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RDH11 fusion protein Ag1485
• Full Name: retinol dehydrogenase 11 (all-trans/9-cis/11-cis)
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC011727
• Gene Symbol: RDH11
• Gene ID (NCBI): 51109
• RRID: AB_2177870
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RDH11(Retinol dehydrogenase 11) is also named as ARSDR1(androgen-regulated short-chain dehydrogenase/reductase 1 ), PSDR1(prostate short-chain dehydrogenase/reductase 1) and belongs to the short-chain dehydrogenases/reductases (SDR) family. RDH11 is involved in steroid synthesis and/or degradation in normal and neoplastic prostate epithelium, and, as such, it may be a key enzyme involved in maintaining intracellular balance of steroid hormones in these cells(PMID:11245473). The deduced protein contains 318 amino acids, and the transcript contains 2 potential polyadenylation signals. It has 2 isoforms produced by alternative splicing.