RDH12 Polyclonal antibody

• 

  WB analysis using 13289-3-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 13289-3-AP (RDH12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse eye using 13289-3-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 13289-3-AP (RDH12 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse eye using 13289-3-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 13289-3-AP (RDH12 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RDH12 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13289-3-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

All-trans and 9-cis retinol dehydrogenase, EC:1.1.1.300, Retinol dehydrogenase 12, SDR7C2, Short chain dehydrogenase/reductase family 7C member 2

---

Tested Applications

• Positive WB detected in: mouse eye tissue
• Positive IHC detected in: mouse eye tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13289-3-AP targets RDH12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3850

  Product name: Recombinant human RDH12 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-316 aa of BC025724

  Sequence: MLVTLGLLTSFFSFLYMVAPSIRKFFAGGVCRTNVQLPGKVVVITGANTGIGKETARELASRGARVYIACRDVLKGESAASEIRVDTKNSQVLVRKLDLSDTKSIRAFAEGFLAEEKQLHILINNAGVMMCPYSKTADGFETHLGVNHLGHFLLTYLLLEQLKVSAPARVVNVSSVAHHIGKIPFHDLQSEKRYSRGFAYCHSKLANVLFTRELAKRLQGTGVTTYAVHPGVVRSELVRHSSLLCLLWRLFSPFVKTAREGAQTSLHCALAEGLEPLSGKYFSDCKRTWVSPRARNNKTAERLWNVSCELLGIRWE

• Full Name: retinol dehydrogenase 12 (all-trans/9-cis/11-cis)
• Calculated Molecular Weight: 316 aa, 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC025724
• Gene Symbol: RDH12
• Gene ID (NCBI): 145226
• RRID: AB_2178131
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96NR8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Retinol dehydrogenase 12 (RDH12) is a member of the short-chain dehydrogenase/reductase (SDR) superfamily. This NADPH-dependent enzyme is highly expressed in the photoreceptor inner segments of the retina, where it functions as a key component of the visual cycle. RDH12 catalyzes the reduction of all-trans-retinal to all-trans-retinol, a critical step in regenerating the visual chromophore 11-cis-retinal. Mutations in the RDH12 gene cause autosomal recessive retinitis pigmentosa (RP) and Leber congenital amaurosis (LCA), severe inherited retinal dystrophies characterized by early-onset vision loss and photoreceptor degeneration. Loss of RDH12 activity leads to accumulation of toxic all-trans-retinal, triggering oxidative stress and cell death. RDH12 also exhibits retinoid-binding properties and may play additional roles in lipid metabolism and cellular stress responses beyond its enzymatic function in the visual cycle.