RDH5 Polyclonal antibody

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  WB analysis using 13140-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13140-1-AP (RDH5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

RDH5 Polyclonal Antibody for WB, ELISA

Cat No. 13140-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

11 cis RDH, 11 cis retinol dehydrogenase, HSD17B9, RDH1, RDH5, SDR9C5

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Tested Applications

• Positive WB detected in: mouse eye tissue, rat eye tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13140-1-AP targets RDH5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3784

  Product name: Recombinant human RDH5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 19-290 aa of BC028298

  Sequence: RDRQSLPASNALVFITGCDSGFGRLLALQLDQRGFRVLASCLTPSGAEDLQRVASSRLHTTLLDITDPQSVQQAAKWVEMHVKEAGLFGLVNNAGVAGIIGPTPWLTRDDFQRVLNVNTMGPIGVTLALLPLLQQARGRVINITSVLGRLAANGGGYCVSKFGLEAFSDSLRRDVAHFGIRVSIVEPGFFRTPVTNLESLEKTLQACWARLPPATQAHYGGAFLTKYLKMQQRIMNLICDPDLTKVSRCLEHALTARHPRTRYSPGWDAKLL

• Full Name: retinol dehydrogenase 5 (11-cis/9-cis)
• Calculated Molecular Weight: 318 aa, 35 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC028298
• Gene Symbol: RDH5
• Gene ID (NCBI): 5959
• RRID: AB_2918031
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92781
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RDH5, also named as RDH1, SDR9C5 and HSD17B9, belongs to short-chain dehydrogenases/reductases (SDR) family. RDH5 catalyzes the oxidation of cis-isomers of retinol in an NAD-dependent manner for the final step in the biosynthesis of 11-cis retinaldehyde. Mutations in this gene are associated with fundus albipunctatus, an autosomal recessive eye disease characterized by stationary night blindness and accumulation of white spots in the retina (PubMed:11675386). The predicted size of this protein is 35 kDa. Some paper have reported that the molecular weight is 32 kDa and could form a dimer of about 60 kDa.