RDM1 Polyclonal antibody

• Featured Product
• KD/KO Validated

RDM1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 20156-1-AP

Synonyms

RAD52 homolog B, RAD52 motif 1, RAD52B, RDM1

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Validation Data Gallery

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  WB analysis of PC-3 using 20156-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20156-1-AP (RDM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 20156-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20156-1-AP (RDM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 20156-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 20156-1-AP (RDM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 20156-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20156-1-AP (RDM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 20156-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20156-1-AP (RDM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 20156-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20156-1-AP (RDM1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 20156-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20156-1-AP (RDM1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 20156-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20156-1-AP (RDM1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 20156-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20156-1-AP (RDM1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of PC-3 using 20156-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 20156-1-AP (RDM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PC-3 cells, human placenta tissue, MCF-7 cells
• Positive IHC detected in: human lung tissue, human brain tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

The immunogen of 20156-1-AP is RDM1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RDM1 fusion protein Ag14077
• Full Name: RAD52 motif 1
• Calculated Molecular Weight: 284 aa, 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC038301
• Gene Symbol: RDM1
• Gene ID (NCBI): 201299
• RRID: AB_10666433
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RDM1, also named as RAD52B, encodes a RNA recognition motif (RRM)-containing protein confered resistance to the antitumor agent cisplatin. The expression of RDM1 is closely related to the cell proliferation and apoptosis of thyroid carcinoma cells and RDM1 may be a potential marker of lung adenocarcinoma. RDM1 has many isoforms (8 kDa, 12-19 kDa and 25-32 kDa) with long or short N-terminus and a great diversity in the exonic composition of C-terminus generated by alternative pre-mRNA splicing and differential usage of two translational start sites. RDM1 protein can be expressed differently in cytoplasm or nuclear/nucleolar in the absence of stress, proteotoxic stress and mild heat shock, suggesting functional differences among the RDM1 proteins. ( PMID: 28939762, 17905820, 28939762, 30069034)

Publications

Species	Application	Title
human	WB,IHC,IF	Mol Oncol; Loss of RDM1 enhances hepatocellular carcinoma progression via p53 and Ras/Raf/ERK pathways.; Authors - Shi-Lu Chen ; KD Validated
human	WB	J Cell Mol Med; RDM1 promotes critical processes in breast cancer tumorigenesis.; Authors - Yajun Chen ; KD Validated
human	WB	Front Cell Dev Biol; A Novel Signature Constructed by RNA-Binding Protein Coding Genes to Improve Overall Survival Prediction of Glioma Patients.; Authors - Zewei Tu
human	WB,IHC	Artif Cells Nanomed Biotechnol; RDM1 plays an oncogenic role in human ovarian carcinoma cells.; Authors - Lu Tong ; KD Validated
human	WB,IHC	Sci Rep; RDM1 plays an oncogenic role in human lung adenocarcinoma cells.; Authors - Lu Tong ; KD Validated
human	WB,IHC	FEBS Open Bio; RDM1 promotes neuroblastoma growth through the RAS-Raf-MEK-ERK pathway.; Authors - Guojin Xie ; KD Validated