REC8 Polyclonal antibody

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• REC8 Polyclonal antibody (10793-1-AP)

REC8 Polyclonal Antibody for WB, ELISA

Cat No. 10793-1-AP Copied!

Publications (15)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Synonyms

Cohesin Rec8p, HR21spB, REC8 homolog (yeast), REC8L1, Rec8p

• 

  WB analysis of human testis using 10793-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10793-1-AP (REC8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 10793-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10793-1-AP (REC8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human testis using 10793-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10793-1-AP (REC8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human testis tissue, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10793-1-AP targets REC8 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1250

  Product name: Recombinant human REC8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 188-548 aa of BC010887

  Sequence: VLPPEAITILEAEPIRMLEIEGERELPEVSRRELDLLIAEEEEAILLEIPRLPPPAPAEVEGIGEALGPEELRLTGWEPGALLMEVTPPEELRLPAPPSPERRPPVPPPPRRRRRRRLLFWDKETQISPEKFQEQLQTRAHCWECPMVQPPERTIRGPAELFRTPTLSGWLPPELLGLWTHCAQPPPKALRRELPEEAAAEEERRKIEVPSEIEVPREALEPSVPLMVSLEISLEAAEEEKSRISLIPPEERWAWPEVEAPEAPALPVVPELPEVPMEMPLVLPPELELLSLEAVHRAVALELQANREPDFSSLVSPLSPRRMAARVFYLLLVLSAQQILHVKQEKPYGRLLIQPGPRFH

• Full Name: REC8 homolog (yeast)
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 85-95 kDa
• GenBank Accession Number: BC010887
• Gene Symbol: REC8
• Gene ID (NCBI): 9985
• RRID: AB_2178282
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95072
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

REC8, also named as Rec8p and REC8L1, belongs to the rad21 family. This cohesin protein is required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II. Rec8 is present in an unphosphorylated form early in meiotic prophase but is phosphorylated prior to meiosis I. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human REC8. This antibody can recognize the monomeric, non-phosphorylated form(63-70kd) of REC8 and other forms(80-100kd) which represent either posttranslationally modified forms or alternative transcript products (PMID: 12615909,19463812).

Publications

What published studies show

The anti-REC8 antibody (Cat# 10793-1-AP) has 15 total citations across journals including Nature Communications, Clinical Cancer Research, Cell Cycle, International Journal of Molecular Sciences, Biological Research, Biomedicine & Pharmacotherapy, Experimental Cell Research, Frontiers in Genetics, Molecular Reproduction and Development, Oncotarget, COPD Journal, Ecotoxicology and Environmental Safety, and Journal of Skin Cancer. Research fields span oocyte meiosis and chromosome segregation, cancer biology (gastric cancer, melanoma, T-cell lymphoma), reproductive endocrinology, and testicular development.

Automatically generated from the 15 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Nat Commun; CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I; Authors - Cheng-Jie Zhou
human	WB	Clin Cancer Res; Ectopic Expression of Cancer-Testis Antigens in Cutaneous T-cell Lymphoma Patients.; Authors - Ivan V Litvinov
mouse	IF	Ecotoxicol Environ Saf; Impaired testicular development in male offspring following gestational DEHP exposure: The role of macrophage-Leydig cell axis.; Authors - Songyi Ye
human	IF	Int J Mol Sci; "Mitotic Slippage" and Extranuclear DNA in Cancer Chemoresistance: A Focus on Telomeres.; Authors - Kristine Salmina
human	IHC	Biol Res; REC8 suppresses tumor angiogenesis by inhibition of NF-κB-mediated vascular endothelial growth factor expression in gastric cancer cells.; Authors - Miao Liu
rat,mouse	WB	Biomed Pharmacother; Ephedra herb reduces adriamycin-induced testicular toxicity by upregulating the gonadotropin-releasing hormone signalling pathway.; Authors - Yang Fu