RECQL5 Polyclonal antibody

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  WB analysis using 12468-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12468-2-AP (RECQL5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 12468-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12468-2-AP (RECQL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 12468-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12468-2-AP (RECQL5 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 12468-2-AP

  Immunofluorescent analysis of HepG2 cells, using RECQL5 antibody 12468-2-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

RECQL5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12468-2-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

EC:5.6.2.4, DNA helicase, RecQ-like type 5, DNA helicase, RecQ like type 5, DNA 3'-5' helicase RecQ5, ATP-dependent DNA helicase Q5

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Tested Applications

• Positive WB detected in: HEK-293T cells, mouse testis tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• CoIP: See 1 publications below

Product Information

12468-2-AP targets RECQL5 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3145

  Product name: Recombinant human RECQL5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC016911

  Sequence: MSSHHTTFPFDPERRVRSTLKKVFGFDSFKTPLQESATMAVVKGNKDVFVCMPTGAGKSLCYQLPALLAKGITIVVSPLIALIQDQVDHLLTLKVRVSSLNSKLSAQERKELLADLEREKPQTKILYITPEMAASSSFQPTLNSLVSRHLLSYLVVDEAHCVSQWGHDFRPDYLRLGALRSRLGHAPCVALTATATPQVQEDVFAALHLKKPVAIFKTPCFRANLFYDVQFKELISDPYGNLKDFCLKALGQEADKGLSGCGIVYCRTREACEQLAIELSCRGVNAKAYHAGLKASERTLVQNDWMEEKVPVIVATISFGMGVDKANVRFVAHWNIAKSMAGYYQESGRA

• Full Name: RecQ protein-like 5
• Calculated Molecular Weight: 435 aa, 49 kDa, 409 kDa
• Observed Molecular Weight: 48 kDa, 130-140 kDa
• GenBank Accession Number: BC016911
• Gene Symbol: RECQL5
• Gene ID (NCBI): 9400
• RRID: AB_2300899
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O94762
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,CoIP	bioRxiv; Senataxin and DNA-PKcs Redundantly Promote Non-Homologous End Joining Repair of DNA Double Strand Breaks During V(D)J Recombination; Authors - Bo-Ruei Chen
human	WB	Adv Sci (Weinh); Specific Monitoring the DNA Helicase Function via Anchor-Embedded DNA Probe; Authors - Keni Ning
human	WB	bioRxiv; Distinct mechanisms underlying extrachromosomal telomere DNA generation in ALT cancers; Authors - Junyeop Lee