REL Monoclonal antibody

REL Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F6A4

Cat no : 67747-1-Ig

Synonyms

C Rel, Proto oncogene c Rel, REL

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Validation Data Gallery

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  WB analysis using 67747-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67747-1-Ig (REL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of HEK-293 using 67747-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67747-1-Ig (REL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Daudi cells, HEK-293 cells, Jurkat cells, MOLT-4 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67747-1-Ig targets REL in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: REL fusion protein Ag26803
• Full Name: v-rel reticuloendotheliosis viral oncogene homolog (avian)
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 68 kDa, 80 kDa
• GenBank Accession Number: NM_001291746
• Gene Symbol: REL
• Gene ID (NCBI): 5966
• RRID: AB_2918516
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

REL, also known as, Proto-oncogene c-Rel, is a 619 amino acid protein, which may play a role in differentiation and lymphopoiesis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The NF-kappa-B heterodimer RELA/p65-c-Rel is a transcriptional activator. The full-length 80-kDa c-Rel was identified (PMID: 9857058).