Validation Data Gallery
|Positive WB detected in
|HeLa cells, MCF-7 cells, Raji cells
|Positive IP detected in
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:1000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
10410-1-AP targets RFC2 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|RFC2 fusion protein Ag0663
|replication factor C (activator 1) 2, 40kDa
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
RFC2, also named as RFC40, belongs to the activator 1 small subunits family. It is a 40kd Activator 1 subunit. It binds ATP. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. RFC2 is a constitutively expressed protein essential for both DNA repair and replication. (PMID: 20660762).
Mol Syst Biol
The MHC I immunopeptidome conveys to the cell surface an integrative view of cellular regulation.
Proc Natl Acad Sci U S A
Deletion of TDP-43 down-regulates Tbc1d1, a gene linked to obesity, and alters body fat metabolism.
RFC2: a prognosis biomarker correlated with the immune signature in diffuse lower-grade gliomas.
Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA polyubiquitination
Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA ubiquitination
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Lei (Verified Customer) (11-29-2018)
This antibody works well in WB. For IP, 50% glycerol contain interferes with antibody-protein A bead binding.