RFC2 Polyclonal antibody

RFC2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 10410-1-AP

Synonyms

A1, A1 40 kDa subunit, Activator 1 40 kDa subunit, Activator 1 subunit 2, Replication factor C subunit 2, RF C 40 kDa subunit, RFC2, RFC40



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, Raji cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 5 publications below

Product Information

10410-1-AP targets RFC2 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RFC2 fusion protein Ag0663
Full Name replication factor C (activator 1) 2, 40kDa
Calculated molecular weight 40 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC002813
Gene symbol RFC2
Gene ID (NCBI) 5982
RRIDAB_2285035
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RFC2, also named as RFC40, belongs to the activator 1 small subunits family. It is a 40kd Activator 1 subunit. It binds ATP. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. RFC2 is a constitutively expressed protein essential for both DNA repair and replication. (PMID: 20660762).

Protocols

Product Specific Protocols
WB protocol for RFC2 antibody 10410-1-APDownload protocol
IF protocol for RFC2 antibody 10410-1-APDownload protocol
IP protocol for RFC2 antibody 10410-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Syst Biol

The MHC I immunopeptidome conveys to the cell surface an integrative view of cellular regulation.

Authors - Caron Etienne E
mouseWB

Proc Natl Acad Sci U S A

Deletion of TDP-43 down-regulates Tbc1d1, a gene linked to obesity, and alters body fat metabolism.

Authors - Chiang Po-Min PM
humanWB

Sci Rep

RFC2: a prognosis biomarker correlated with the immune signature in diffuse lower-grade gliomas.

Authors - Xu Zhao
humanWB

bioRxiv

Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA polyubiquitination

Authors - Yi-Chang Wang
humanWB

Cell Rep

Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA ubiquitination

Authors - Yi-Chang Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Lei (Verified Customer) (11-29-2018)

This antibody works well in WB. For IP, 50% glycerol contain interferes with antibody-protein A bead binding.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HepG2
RFC2 Antibody Western Blot, validation (1:500 dilution) in HepG2 (Cat no:10410-1-AP)