RFC4 Polyclonal antibody
RFC4 Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 10806-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MCF-7 cells, LNCaP cells, Raji cells |
Positive IF/ICC detected in | U2OS cells, HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 3 publications below |
Product Information
10806-1-AP targets RFC4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RFC4 fusion protein Ag1234 |
Full Name | replication factor C (activator 1) 4, 37kDa |
Calculated Molecular Weight | 37 kDa |
Observed Molecular Weight | 37-40 kDa |
GenBank Accession Number | BC017452 |
Gene Symbol | RFC4 |
Gene ID (NCBI) | 5984 |
RRID | AB_2178477 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for RFC4 antibody 10806-1-AP | Download protocol |
IF protocol for RFC4 antibody 10806-1-AP | Download protocol |
FC protocol for RFC4 antibody 10806-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Aging (Albany NY) The upregulated expression of RFC4 and GMPS mediated by DNA copy number alteration is associated with the early diagnosis and immune escape of ESCC based on a bioinformatic analysis. | ||
Cell Rep Cyclin N-Terminal Domain-Containing-1 Coordinates Meiotic Crossover Formation with Cell-Cycle Progression in a Cyclin-Independent Manner. | ||