RFFL Polyclonal antibody

• Featured Product
• KD/KO Validated

RFFL Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12687-1-AP

Synonyms

CARP 2, Caspase regulator CARP2, Fring, RFFL, RIFIFYLIN, RING finger protein 189, RING finger protein 34 like, RNF189, RNF34L

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Validation Data Gallery

• 

  WB analysis of mouse testis using 12687-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12687-1-AP (RFFL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 12687-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12687-1-AP (RFFL antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12687-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12687-1-AP (RFFL antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 12687-1-AP

  IP result of anti-RFFL (IP:12687-1-AP, 4ug; Detection:12687-1-AP 1:300) with mouse testis tissue lysate 3520 ug.

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Tested Applications

• Positive WB detected in: mouse testis tissue, mouse thymus tissue
• Positive IP detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• CoIP: See 1 publications below

Product Information

12687-1-AP targets RFFL in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RFFL fusion protein Ag3380
• Full Name: ring finger and FYVE-like domain containing 1
• Calculated Molecular Weight: 327 aa, 37 kDa
• Observed Molecular Weight: 37-41 kDa
• GenBank Accession Number: BC028424
• Gene Symbol: RFFL
• Gene ID (NCBI): 117584
• RRID: AB_10644289
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Biol Chem; Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome; Authors - Hana Nůsková
human	WB,CoIP	Cell Death Dis; ETS-1-activated LINC01016 over-expression promotes tumor progression via suppression of RFFL-mediated DHX9 ubiquitination degradation in breast cancers; Authors - Ying Sun ; KD Validated